Reinnervation of Paralyzed Limb Muscle by Nerve-Muscle-Endplate Grafting Technique

reportActive / Technical Report | Accesssion Number: AD1210237 | Open PDF

Abstract:

During the past year, we conducted experiments on animals in the 3-month delayed reinnervation groups (i.e., DelNN, Del-ENF/NN, Del-SP/NN, Del-ANG, and Del-EEA) and 6-month denervation control. Three months aftertreatment, the extent of functional recovery of the treated TA muscles was examined by measuring toe spread distances and maximal muscle force. The extent of neuromuscular recovery was examined by quantifying regenerated axons, reinnervated motor endplates (MEPs), muscle weight, and denervated/atrophied muscle fibers. Our results showed that Del-ENF/NN and Del-SP/NN resulted in better muscle force recovery (72 percent and 70 percent of the control, respectively) than Del-NN alone (59 percent), Del-ANG (38 percent) and Del-EEA (43 percent). Tissue studies showed that Del-ENF/NN and Del-SP/NN resulted in better axonal regeneration, MEP reinnervation, and muscle mass preservation as compared with other delayed reinnervation groups used in this research. These findings suggest that focal injection of ENF and SP procedure are useful therapies for delayed muscle reinnervation.

Author(s):
Mu, Liancai ; Chen, Jingming ; Li, Jing ; Sobotka, Stanislaw ; Nyirenda, Themba
Author Organization(s):
Hackensack Meridian Health, Center for Discovery and Innovation
Funding Organization(s):
ARMY MEDICAL RESEARCH AND DEVELOPMENT COMMAND FORT DETRICK MD, FORT DETRICK, MD
Document Type:
Technical Report/Final Report
Publication Date:
2023 Aug 01
Pagination:
49

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DOCUMENT & CONTEXTUAL SUMMARY

Distribution Code:
A - Approved For Public Release
Distribution Statement: Public Release.
Copyright: Not Copyrighted

RECORD

Collection: TRECMS
Subject Terms
Descriptor(s):
health services, medical personnel, peripheral nervous system, laboratory animals, parkinson's disease, electronic mail, surgery, skeletal muscle, neuropathy, feasibility studies, muscle fibers, sciatic nerve, standards, cameras, cells, data analysis
Keyword(s):
Peripheral nerve injury, limb reinnervation, nmeg(nerve-muscle-endplate grafting), nmz(native motor zone), axonal regeneration, axon-endplate connection, functional recovery
Subject Categories:
Biological and Medical Sciences; Biological and Medical Sciences
Descriptor(s):
health services, medical personnel, peripheral nervous system, laboratory animals, surgery, skeletal muscle, united states, biomedical research, feasibility studies, muscle fibers, sciatic nerve
Creation Date:
2023 Sep 12
Update Date:
2023 Nov 14