mRNA Vaccine for Peanut Allergy

reportActive / Technical Report | Accesssion Number: AD1202710 | Open PDF

Abstract:

During Year 1 of this Discovery Award, we have made significant progress on Specific Aim 1. We provided peanut allergen sequence information to Synbio Technologies who then synthesized Ara h 1, 2, 3 and 6 mRNA, as well as mouse IL-12. We transfected HEK 293T cells with the Ara h 1-6 mRNAs and confirmed protein expression by Western Blot with plasma from peanut-allergic subjects. Cell supernatants and lysates have been sent to MS Bioworks for mass spectrometry analysis of protein expression levels. Once the protein expression has been confirmed by mass spectrometry, we will formulate the mRNA in lipid nanoparticles for intramuscular delivery in mice, and we will send the mRNA to Orlance for formulation on gold microbeads for Gene Gun delivery in mice. Overall, our data demonstrates that peanut allergen mRNA can be expressed in mammalian cells, indicating that we are making progress towards the mRNA vaccine.

Author(s):
Kulis, Michael
Author Organization(s):
NORTH CAROLINA UNIV AT CHAPEL HILL
Funding Organization(s):
ARMY MEDICAL RESEARCH AND DEVELOPMENT COMMAND FORT DETRICK MD, FORT DETRICK , MD
Document Type:
Technical Report/Annual Report
Publication Date:
2023 Mar 01
Pagination:
8

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution Code:
A - Approved For Public Release
Distribution Statement: Public Release

RECORD

Collection: TRECMS
Identifying Numbers
Grant Number(s):
W81XWH-22-1-0066
Subject Terms
Communities of Interest:
Biomedical
Descriptor(s):
mass spectrometry, biomedical research, mrna vaccines, north carolina, nucleic acids, spectrometry, students, mrna, vaccines, allergens, maryland, professional development, technology transfer, department of defense, experimental design, information operations, intramuscular injections
Keyword(s):
Food allergy, peanut allergy, mRNA vaccine, Gene Gun, rna(ribonucleic acids), Ara h 1, Ara h 2, Ara h 3, Ara h 6, IgG
Subject Categories:
Biological and Medical Sciences
Creation Date:
2023 Jun 06
Update Date:
2023 Jul 20