Direct Imaging of Modulatory Neurotransmitters Using Synthetic Nanosensors to Understand and Treat Parkinson's Disease

reportActive / Technical Report | Accesssion Number: AD1155695 | Open PDF

Abstract:

Nanosensors for the optical detection of dopamine in brain slice has been validated in wildtype C57/C6 mice as well as a Parkinson's model mouse. Using nanosensors, endogenous dopamine release in striatal brain slice is triggered by electrical stimulation and quantified using microscopy. We have established the workflow for this procedure and have prepared a population of Parkinson's and wildtype mice to image in the coming quarter. We have confirmed that the method can quantitatively distinguish dopamine release between sub-regions of the striatum, a critical step in confirming the method for use in Parkinson's studies. COVID-19 shutdowns stalled research starting in Mar. Research facilities are reopening and the project is expected to resume this quarter.

Author(s):
Del Bonis-o'donnell, Jackson
Author Organization(s):
Regents of the University of California
Funding Organization(s):
U.S. Army Medical Research and Development Command, Fort Detrick , MD
Document Type:
Technical Report/Final Report
Publication Date:
2021 May 01
Pagination:
17

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution Code:
A - Approved For Public Release
Distribution Statement: Public Release

RECORD

Collection: TRECMS
Identifying Numbers
Grant Number(s):
W81XWH-18-1-0282
Subject Terms
Modernization Areas:
Microelectronics
Communities of Interest:
Biomedical
Descriptor(s):
parkinson's disease, medical personnel, brain, neurons, carbon nanotubes, diseases and disorders, research facilities, biomedical research, california, data analysis, governments, public health, students, therapy, covid-19, detectors, dopamine
Keyword(s):
Fluorescence imaging, Sensing, Neuromodulators, dyskinesia
Subject Categories:
Biological and Medical Sciences
Creation Date:
2021 Dec 27
Update Date:
2022 Feb 01