Understanding the 'Mission Versatility' of Membrane Proteins via Nanoscopic Imaging

reportActive / Technical Report | Accesssion Number: AD1155537 | Open PDF

Abstract:

The goal of this AFOSR Young Investigator Program grant is to investigate the nanoscale structural and functional dynamics of membrane proteins in their native liquid environment using liquid phase transmission electron microscopy (TEM). Over the project time period, we optimized the self-assembly and purification of nanodiscsmembrane proteins in a single-particle form amenable to TEMobservationcontaining the protein cytochrome p450 2J2 (CYP-2J2) and then developed a graphene liquid sandwich to encapsulate CYP- 2J2 nanodiscs in their native buffer conditions for imaging.

Author(s):
Chen, Qian
Author Organization(s):
UNIVERSITY OF ILLINOIS
Funding Organization(s):
AFOSR, ARLINGTON , VA
Document Type:
Technical Report/Final Report
Publication Date:
2021 Sep 30
Pagination:
10

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution Code:
A - Approved For Public Release
Distribution Statement: Public Release

RECORD

Collection: TRECMS
Identifying Numbers
Report Number(s):
AFRL-AFOSR-VA-TR-2022-0056
Contract Number(s):
FA9550-17-1-0296
Program Element Number(s):
PE61102F
Subject Terms
Modernization Areas:
Microelectronics
Communities of Interest:
Advanced Electronics
Descriptor(s):
molecular dynamics, chemistry, electron microscopy, dynamics, liquid phases, membrane proteins, microscopy, transmission electron microscopy, boundaries, chemical analysis, engineering, lipids, liquid chromatography, proteins, self assembly, statistical mechanics, universities
Subject Categories:
Biological and Medical Sciences; Physics
Creation Date:
2021 Dec 21
Update Date:
2022 Jan 24