Improving Protein Stability and Activity Using Zwitterionic Peptides

reportActive / Technical Report | Accesssion Number: AD1058598 | Open PDF

Abstract:

This work established the use of polypeptides composed of repeating glutamic acid (E) and lysine (K) (poly(EK)) for the purpose of increasing protein stability. The addition of poly(EK) to the primary sequence at the C-terminus of a model beta-lactamase protein and a destabilized TEM-19 demonstrated significant ability in increasing protein stability while maintaining bioactivity. The use of fusion protein products allows for a one-step protocol resulting in a homogeneous product, a significant advantage over polymer-based approaches.

Author(s):
Jiang, Shaoyi
Author Organization(s):
University of Washington
Funding Organization(s):
Publication Date:
2017 Mar 13
Pagination:
0

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RECORD

Collection: TRECMS
Subject Terms
Communities of Interest:
No COI(s) Identified
Descriptor(s):
chemistry, molecular dynamics, engineering, genetic engineering, chemical synthesis, glutamic acid, department of defense, mathematics, polymers, sequences, solid phases, therapy, abstracts, agreements, materials, proteins, students
Keyword(s):
Fusion proteins, EK peptides, zwitterionic peptides, polypeptides, e(glutamic acid), k (lysine), poly(EK)
Subject Categories:
Biological and Medical Sciences; Biological and Medical Sciences
Update Date:
2018 Sep 05