Photo-Oxidation From Mode-Locked Laser Exposure to hTERT-RPE1 Cells

reportActive / Technical Report | Accession Number: ADP020036 | Open PDF

Abstract:

Human retinal pigment epithelial RPE cells hTERT-RPE1 were used to detect photo-oxidation products generated from chronic NIR 810 nm laser exposure. Exposure of a discrete area within cell monolayers provided a means of distinguishing fluorescence above background levels. Oxidative stress was detected using the fluorescent dye H2DCF-DA and its analog CM-H2DCF-DA. Fluorescence was detected in cells exposed to mode-locked 76 MHz, -160 femtoseconds but not CW laser exposure. Detection of photo- oxidation from the mode-locked laser was dependent upon radiant exposure, but only if irradiance was greater than a threshold value. The CM-H2DCF-DA dye proved a more sensitive indicator of oxidation than H2DCF-DA, and the radiant exposure threshold for detection was dependent upon dye concentration. No oxidation was detected from CW exposures using the most sensitive fluorescent dye conditions when using 3 times the irradiance, and 10 times the radiant exposure needed to detect fluorescence from mode- locked exposure.

Author(s):
Denton, Michael L. ; Eikum, Debbie M. ; Noojin, Gary D. ; Stolarski, David J. ; Glickman, Randolph D. ; Rockwell, Benjamin A.
Author Organization(s):
NORTHROP GRUMMAN CORP SAN ANTONIO TX INFORMATION TECHNOLOGY
Descriptive Note:
Conference paper
Supplementary Note:
Presented at Laser Interaction with Tissue and Cells XV held in San Jose, CA on 26-28 Jan 2004. Pub. in Proceedings of SPIE, v5319, Progress in Biomedical Optics and Imaging, v5 n8, p231-237, 2004. ISSN-1-605-7422, ISBN-0-8194-5227-0. This article is from ADA436676 Laser Interaction with Tissue and Cells XV. Held in San Jose, Ca on 26-28 January 2004.
Pagination:
0007

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release
Distribution Statement:
Approved For Public Release; Distribution Is Unlimited.

RECORD

Collection: TR
Identifying Numbers
Monitor Series:
AFOSR
 Subject Terms
Modernization Areas:
Directed Energy
Communities of Interest:
Biomedical
Descriptor(s):
*EPITHELIUM, *OXIDATION, *RETINA, *PIGMENTS, *MODE LOCKED LASERS, *LASER DAMAGE, *CELLS(BIOLOGY), FLUORESCENCE, PHOTOCHEMICAL REACTIONS, CONTINUOUS WAVE LASERS, LASER HAZARDS, FLUORESCENT DYES, EXPOSURE(PHYSIOLOGY), HUMANS, THRESHOLD EFFECTS, SENSITIVITY
Field(s)/Group(s):
Stress Physiology, Lasers and Masers
Keyword(s):
*HTERT RPE1 CELLS, COMPONENT REPORTS, RPE(RETINAL PIGMENT EPITHELIAL), HTERT RPE1(HUMAN RETINAL PIGMENT EPITHELIAL RETINAL PIGMENT EPITHELIAL CELLS), *PHOTOOXIDATION, PHOTOOXIDATIVE STRESS
Report Date:
2004 Jan 01
Creation Date:
2006 Feb 15