Antagonist and Agonist Activities of Synthetic Peptide Fragments of g-CSF and their Protein Conjugates,

Granulocyte-colony stimulating factor g-CSF is an 18-22 kDa glycoprotein which specifically stimulates the formation of granulocyte progenitor colonies in vitro and increases neutrophil, monocyte, and eosinophil populations in a dose-dependent manner in vitro. In an epitope-mapping study undertaken in our laboratories, a Hopp and Woods hydropathic profile of g-CSF identified a series of peptide fragments as likely candidates for generating g-CSF cross-reactive antibodies.