Characterization of Nanomaterials Using Field Flow Fractionation and Single Particle Inductively Coupled Plasma Mass Spectrometery (FFF-ICP-MS and SP-ICP-MS): Scientific Operating Procedure SOP-C

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Abstract:

Characterization of nanomaterials must include analysis of both size and chemical composition. Field Flow Fractionation FFF is a powerful tool for determining the size of nanoparticles. Through the use of a combination of common detectors, such as UV-VIS Ultraviolet-Visible Spectrophotometry absorbance, with advanced methods, such as ICP-MS Inductively coupled plasma mass spectrometry, high-resolution nanoparticle sizing and compositional analysis at the mgL concentration level can be obtained. Single particle counter ICP-MS SP-ICP-MS has increased sensitivity compared to Field Flow Fractionation Inductively coupled plasma mass spectrometry FFF-ICP-MS, with detection and sizing concentrations of ngL. Such low-level detection and characterization capability is critical to nanomaterial investigations at biologically and environmentally relevant concentrations. The techniques have been modified and applied to characterization of all four elemental constituents of Cadmium SelenideZinc Sulfide core-shell quantum dots, silver nanoparticles with gold seed cores, and gold nanoparticles. Additionally, sulfide coatings on silver nanoparticles can be detected as a potential means to determine environmental aging of nanoparticles. Extraction of nanoparticles from tissues is possible using tetramethylammonium hydroxide TMAH. Though any analysis described above is possible, only SP-ICP-MS has been employed to detect tissue extracts. This SOP Scientific Operating Procedure describes the analysis of engineered nanoparticles ENPs, through the various separation and detection techniques described above. These analytical tools were tested on a variety of gold and silver standard nanoparticles that have been extensively characterized.

Author(s):
Bednar, Anthony J. ; Poda, Aimee R. ; Kennedy, Alan J. ; Armstrong, Kristie C. ; Gray, Evan P. ; Higgins, Christopher ; Ranville, James F.
Author Organization(s):
ENGINEER RESEARCH AND DEVELOPMENT CENTER VICKSBURG MS ENVIRONMENTAL LAB
Descriptive Note:
Final rept.
Supplementary Note:
The original document contains color images. Prepared in collaboration with the Department of Civil and Environmental Engineering and Department of Chemistry and Geochemistry, Colorado School of Mines, Golden.
Pagination:
0037

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release
Distribution Statement:
Approved For Public Release; Distribution Is Unlimited.

RECORD

Collection: TR
Identifying Numbers
Report Number(s):
ERDC/EL-SR-15-3
Monitor Series:
USACE
 Subject Terms
Joint Capability Areas:
JCA_5_Command and Control; JCA_5.3_Planning; JCA_1.3.2_Personnel Management; JCA_4.5.4_Requirements Definition; JCA_4.5_Operational Contract Support; JCA_JCA_4.1.2_Sustain the Force; JCA_4.1_Deployment and Distribution; JCA_4.6_Engineering
Communities of Interest:
No COI(s) Identified
Descriptor(s):
*FRACTIONATION, *MASS SPECTROMETRY, *NANOMATERIALS, *SPECTROPHOTOMETRY, CADMIUM SELENIDES, CHEMICAL COMPOSITION, COATINGS, CONCENTRATION(COMPOSITION), FLOW, GOLD, HYDROXIDES, NANOPARTICLES, NANOTECHNOLOGY, PARTICLE SIZE, QUANTUM DOTS, SAMPLING, SILVER, ZINC SULFIDES
Field(s)/Group(s):
Industrial Chemistry and Chemical Processing, Physical Chemistry, Miscellaneous Materials, Manufacturing and Industrial Engineering and Control of Production Systems, Nuclear Physics and Elementary Particle Physics
Keyword(s):
ENP(ENGINEERED NANOPARTICLES), FFF(FIELD FLOW FRACTIONATION), ICP MS(INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY), NANOPARTICLE CHARACTERIZATION, UV VIS(ULTRAVIOLET VISIBLE SPECTROPHOTOMETRY), SP(SINGLE PARTICLES), *FFF ICP MS, *SP ICP MS, TMAH(TETRAMETHYLAMMONIUM HYDROXIDE)
Report Date:
2015 Apr 01
Creation Date:
2015 Apr 20