Infection of the Gulf Coast Tick, Amblyomma Maculatum (Acari: Ixodidae), with Rickettsia Parkeri: First Report from the State of Delaware

reportActive / Technical Report | Accession Number: ADA580052 | Open PDF

Abstract:

The molecular detection of Rickettsia parkeri in a Gulf Coast tick, Amblyomma maculatum, collected in Delaware represents the first evidence of the human pathogen R. parkeri associated with A. maculatum in the state. A total of five adult 2 male and 3 female Gulf Coast ticks were collected from tick drags conducted during a two-day sampling event 21 22 May 2012 at Bombay Hook National Wildlife Refuge, near Smyrna, Delaware. All specimens were tested for the presence of Rickettsia with a genus-specific quantitative real-time polymerase chain reaction qPCR assay one of the female specimens tested positive. This specimen was then assessed for the presence of Rickettsia parkeri and Candidatus Rickettsia andeanae by two species-specific qPCR assays. The presence of R. parkeri DNA was detected, whereas Candidatus R. andeanae DNA was not.

Author(s):
Florn, David A. ; Jiang, Ju ; Robbins, Richard G. ; Richards, Allen L.
Author Organization(s):
NAVAL MEDICAL RESEARCH CENTER SILVER SPRING MD RICKETTSIAL DISEASES DEPT
Descriptive Note:
Journal article
Supplementary Note:
Published in Systematic & Applied Acarology, v18 n1 p27 29, 31 Mar 2013. Prepared in collaboration with the Uniformed Services University of the Health Sciences, Department of Preventive Medicine and Biometrics, Bethesda, MD and the Armed Forces Pest Management Board, Office of the Deputy Under Secretary of Defense for Installations and Environment, Silver Spring, MD. Sponsored in part by the DoD AFHSC/GEIS.
Pagination:
0004

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release
Distribution Statement:
Approved For Public Release; Distribution Is Unlimited.

RECORD

Collection: TR
Identifying Numbers
Contract/Grant Number(s):
R087M6
Monitor Series:
USUHS
 Subject Terms
Joint Capability Areas:
JCA_6.4.1_Secure Information Exchange; JCA_1.4.1_Force Health Protection; JCA_1.4_Force Support; JCA_6.4_Information Assurance; JCA_6_Net Centric; JCA_8_Building Partnerships
Communities of Interest:
Biomedical
Descriptor(s):
*RICKETTSIA, *TICKS, DELAWARE, DEOXYRIBONUCLEIC ACIDS, GENES, INFECTIOUS DISEASES, PATHOGENESIS, PROTEINS
Field(s)/Group(s):
Biology, Microbiology
Keyword(s):
AMBLYOMMA MACULATUM, RICKETTSIA PARKERI, GULF COAST TICKS, MOLECULAR DETECTION, QPCR(QUANTITATIVE REAL TIME POLYMERASE CHAIN REACTIONS), WU0000188M 0931 001 A0074
Report Date:
2013 Mar 31
Creation Date:
2013 Jul 24