The Use of Comprehensive Molecular Profiling with Network and Control Theory to Better Understand GWI and Model Therapeutic Strategies

The objective of this study is to improve our understanding of GWI pathogenesis in two ways through integration across several of the body s regulatory systems of data and knowledge collected from disparate sources, and by mapping of the coordinated interactions between these physiologic systems and the potential for altered wiring of these signaling networks in GWI. Using comprehensive molecular profiling, network and control theory the overarching objective of this proposal is to define the precise nature of these irregularities in immune and neuroendocrine signaling as well as the altered activation states of the corresponding cells such that treatment courses can be designed to redirect the system as a whole to normal pattern of coordinated activity. Recruitment is ongoing and as of July 18, 2011, twenty-two patients have been consented to participate in the study sixteen of the subjects recruited are symptomatic with GWI, four of the subjects are healthy controls and two of the subjects are screen failures. There was a delay in initiating the exercise challenge component of the study due to local requirements, documenting and approvals through several committees on the standard operating procedures for the sterilization of our reusable equipment which consist of a mask attached to the equipment that measures expired gases. This protocol s initial subject assessment was delayed while we attempted to expedite this process. The sterilization process was approved by the Infectious Control Committee and the Reusable Medical Equipment committee as of June 23, 2010 and Exercise Challenges commenced in August 2010. Currently, subjects are being recruited by the use of many different measures. Emphasis has been placed in attracting health Gulf War Veterans during the seond half of the annual term.