Exploring Neurofibromin Function in a Yeast Model of NF1

reportActive / Technical Report | Accession Number: ADA554095 | Open PDF

Abstract:

We hypothesized that the NF1 protein Neurofibromin has functions in addition to its Ras-GAP activity and proposed to develop a yeast NF1 model to define new functions as well as novel regulators of known functions. In year 1 of this project, we categorized genetic hits that we recovered from yeast screens. We divided hits into Ras-dependent and Ras-independent categories. In year 2 of this project, we have identified proteins that physically interact with IRA1 and IRA2 the yeast homologs of human Neurofibromin. These data will facilitate future years of funding for this project, as we integrate the genetic and physical interaction data in order to get a better picture of the cellular pathways that Ira1 and Ira2 regulate, and then to validate if these interactions are relevant in animal models of the disease.

Author(s):
Gitler, Aaron
Author Organization(s):
PENNSYLVANIA UNIV PHILADELPHIA
Descriptive Note:
Annual rept. 1 Sep 2009-31 Aug 2010
Supplementary Note:
The original document contains color images. DOI: 10.21236/ADA554095
Pagination:
0010

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release
Distribution Statement:
Approved For Public Release; Distribution Is Unlimited.

RECORD

Collection: TR
Identifying Numbers
Contract/Grant Number(s):
W81XWH-08-1-0538
Monitor Series:
USAMRMC
 Subject Terms
Joint Capability Areas:
JCA_5_Command and Control
Communities of Interest:
No COI(s) Identified
Descriptor(s):
*PROTEINS, *YEASTS, GENES, MASS SPECTROMETRY, PURIFICATION
Field(s)/Group(s):
Biochemistry, Biology
Keyword(s):
GTPASE, HEAT SHOCK, IRA1 GENE, IRA2 GENE, NEUROFIBROMIN, NF1 GENE, RAS, STATIONARY PHASE, TAP
Report Date:
2010 Sep 01
Creation Date:
2012 Feb 08