Connective Tissue Growth Factor (CTGF) as a Regulator of Lactogenic Differentiation

reportActive / Technical Report | Accession Number: ADA539529 | Open PDF

Abstract:

The degree of differentiation of mammary epithelial cells is dependent on their response to basement membrane and stromal protein-induced signals. Connective Tissue Growth Factor CTGFCCN2, a known stromal mediator, is highly upregulated by dexamethasone and is required for the lactogenic differentiation of mouse mammary epithelial cells. Using a Tet-off system to overexpress CTGFCCN2 in the HC11 cell background, we found that its elevated expression enhanced multiple markers of lactogenic differentiation, including beta-casein transcription, mammosphere formation, and stabilization of Stat5 activity. Elevated levels of CTGFCCN2 partially abrogated the requirement of matrix proteins for the transcription of beta-casein, suggesting that CTGFCCN2 contributes to lactogenic differentiation through a regulation of cellmatrix adhesion.

Author(s):
Morrison, Bethanie L.
Author Organization(s):
UNIFORMED SERVICES UNIV OF THE HEALTH SCIENCES BETHESDA MD F EDWARD HEBERT SCHOOL OF MEDICINE
Descriptive Note:
Doctoral thesis
Pagination:
0178

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release
Distribution Statement:
Approved For Public Release; Distribution Is Unlimited.

RECORD

Collection: TR
Identifying Numbers
Monitor Series:
USUHS
 Subject Terms
Joint Capability Areas:
JCA_5_Command and Control; JCA_5.3_Planning; JCA_1.3.2_Personnel Management; JCA_1.2.5_Lessons Learned; JCA_4.3_Maintain; JCA_1.2.7_Experimentation; JCA_1.2.3_Educating; JCA_4.5.4_Requirements Definition; JCA_4.5_Operational Contract Support
Communities of Interest:
No COI(s) Identified
Descriptor(s):
*CONNECTIVE TISSUE, MEMBRANES(BIOLOGY), THESES, MAMMARY GLANDS, GROWTH(PHYSIOLOGY), EPITHELIUM, CELLS(BIOLOGY)
Field(s)/Group(s):
Anatomy and Physiology
Keyword(s):
CTGF(CONNECTIVE TISSUE GROWTH FACTOR)
Report Date:
2009 Jun 09
Creation Date:
2011 Apr 13