Development of the Electrochemical Biosensor for Organophosphate Chemicals Using CNT/Ionic Liquid Bucky Gel Electrode

Organophosphorus hydrolase OPH immobilized on CNTionic liquid IL electrodes were prepared by using three different intrinsic kinds of ILs, as binders. CNTsILs lead to dramatic electrochemical enhancements with respect to response time, stability, and sensitivity of composite electrodes. In addition, the electrochemical and biocatalytic properties of three-composite electrodes were strongly influenced by different types of ILs used, as verified by cyclic voltammetry and chronoamperometry. These results were attributed to the conformational changes of the microenvironment between the OPH and the composite electrodes within three different types of ILs. In particular, the biocatalytic signals of three OPHCNTILs modified electrodes increased linearly to the concentration of paraoxon in a wide range of 220 lM. These findings provide a deep understanding of the role of each IL on the modified electrodes, enabling to enhance electrochemical properties for biosensors.