The Role of ABC Proteins in Drug Resistant Breast Cancer Cells

reportActive / Technical Report | Accession Number: ADA486167 | Open PDF

Abstract:

This predoctoral training grant, supported by the Department of Defense Breast Cancer Research Program BCRP of the Office of the Congressionally Directed Medical Research Programs CDMRP, aims to study the possible role of ABC transporters in pleitropic drug resistance, using a combination of molecular biological, biochemical, and biophysical methods. The second year of funding was spent working with a member of a drugmetabolite transporter superfamily called the Plasmodium falciparum Chloroquine Transporter PfCRT. While PfCRT is known to be the main molecular determinant of chloroquine resistance, there is only indirect proof of its interaction with the drug. Therefore, I have employed a methodology commonly applied to drug resistance proteins such as human P-glycoprotein and labeled PfCRT with a photoaffinity drug analogue. A manuscript is currently in preparation detailing my results.

Author(s):
Lekostaj, Jacqueline K.
Author Organization(s):
GEORGETOWN UNIV WASHINGTON DC
Descriptive Note:
Annual summary rept. 15 Mar 2006-14 Mar 2008
Supplementary Note:
DOI: 10.21236/ADA486167
Pagination:
0034

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release
Distribution Statement:
Approved For Public Release; Distribution Is Unlimited.

RECORD

Collection: TR
Identifying Numbers
Contract/Grant Number(s):
W81XWH-06-1-0454
Monitor Series:
USAMRMC
 Subject Terms
Joint Capability Areas:
JCA_5_Command and Control; JCA_5.3_Planning; JCA_1.2.3_Educating; JCA_1.2.5_Lessons Learned; JCA_1.2.7_Experimentation; JCA_1.3.2_Personnel Management; JCA_1.2.1_Training; JCA_JCA_4.1.2_Sustain the Force; JCA_4.1_Deployment and Distribution; JCA_5.2.2_Develop Knowledge and Situational Awareness; JCA_5.2_Understand
Modernization Areas:
Biotechnology
Communities of Interest:
No COI(s) Identified
Descriptor(s):
*BREAST CANCER, *PROTEINS, DRUG TOLERANCE, RESISTANCE(BIOLOGY), PLASMODIUM FALCIPARUM, CELLS(BIOLOGY)
Field(s)/Group(s):
Biochemistry, Medicine and Medical Research
Keyword(s):
PFCRT(PLASMODIUM FALCIPARUM CHLOROQUINE TRANSPORTER), DRUG RESISTANCE, DRUG TRANSPORTERS, PHOTOAFFINITY LABELING, HETEROLOGOUS EXPRESSION
Report Date:
2008 Apr 01
Creation Date:
2008 Sep 24