Real-Time Fluorogenic Polymerase Chain Reaction (PCR) Assays to Nucleic Acid-Based Detection of Simulants and Biothreat Agents

reportActive / Technical Report | Accession Number: ADA483954 | Open PDF

Abstract:

Polymerase chain reaction PCR is an exquisitely sensitive method for the amplification and detection of genetic material DNA and RNA sequences. We have developed several assays using a variation of PCR that proceeds very quickly and allows the monitoring of the progress of the reaction in real time. We describe here assays for the detection of the gene encoding staphylococcal enterotoxin A SEA, a toxin produced by the bacterium Staphylococcus aureus and an agent responsible for a significant fraction of food poisoning incidents worldwide. We also describe several assays for the detection of a simulant of viral pathogens, the bacteriophage MS2.

Author(s):
Khan, Akbar S. ; O'connell, Kevin P. ; Bucher, Jennifer R. ; Anderson, Patricia E. ; Cao, Cheng J. ; Gostomski, Mark V. ; Valdes, James J.
Author Organization(s):
ARMY SOLDIER AND BIOLOGICAL CHEMICAL COMMAND NATICK MA
Descriptive Note:
Conference paper
Supplementary Note:
See also ADM001523.
Pagination:
0008

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release
Distribution Statement:
Approved For Public Release; Distribution Is Unlimited.

RECORD

Collection: TR
Identifying Numbers
Monitor Series:
ECBC
 Subject Terms
Joint Capability Areas:
JCA_8_Building Partnerships; JCA_8.2_Shape; JCA_8.2.2_Provide Aid to Foreign Partners and Institutions; JCA_3.2_Engagement; JCA_3_Force Application; JCA_1.2.7_Experimentation; JCA_5_Command and Control; JCA_6.3.3_Spectrum Management; JCA_6_Net Centric; JCA_3.2.2_Non-Kinetic Means
Communities of Interest:
No COI(s) Identified
Descriptor(s):
*STAPHYLOCOCCUS AUREUS, *ENTEROTOXINS, *NUCLEIC ACIDS, *BIOLOGICAL AGENTS, *TOXINS AND ANTITOXINS, *DEOXYRIBONUCLEIC ACIDS, *POLYMERIZATION, CHEMICAL AGENT SIMULANTS, FOOD POISONING, STAPHYLOCOCCUS, CHAIN REACTIONS, RIBONUCLEIC ACIDS, GENES, CODING, BACTERIA, REAL TIME, DETECTION
Field(s)/Group(s):
Toxicology, Microbiology, Polymer Chemistry
Report Date:
2003 Jul 01
Creation Date:
2008 Aug 06