Fluoride Ion Regeneration of Cyclosarin (GF) From Minipig Tissue and Fluids Following Whole Body GF Vapor Exposure

reportActive / Technical Report | Accession Number: ADA481857 | Open PDF

Abstract:

Recent developments to improve nerve agent biomarker techniques include methods for measuring fluoride regenerated Sarin GB in blood and tissue. Our efforts extend the fluoride ion regeneration method to be able to determine cyclosarin GF in red blood cells, plasma, and tissue of minipig blood samples after whole body exposure to GF at miosis levels. Blood samples were taken serially before, during, and after whole body GF exposure from the minipig via venous catheter allowing agent exposure profiles to be generated. After processing the samples with fluoride ion and extracting with C-18 solid phase extraction cartridges the ethyl acetate extract was analyzed by GCMS. The GCMS method utilized an autoinjector, a large volume injector port LVI, positive ion ammonia chemical ionization detection in the SIM mode, and a 2H11-GF stable isotope internal standard. Results indicated that the method range was 10-1000 pg on column. The detection limit was 3 pg of GF on column despite the complexity of the red blood celltissue matrix. Conditions that needed to be optimized for the LVI included injection volume, initial temperature, pressure, and flow rate. The regenerated GF R-GF profiles differ greatly from the regenerated GB R-GB profiles in the minipig at similar exposure levels. The onset of the appearance of R-GF in the blood seems to be delayed and maximum levels are reached at much later times as compared to GB exposures. The rate of R-GB production was 5- 10 times greater than that of R-GF at equimolar exposures.

Author(s):
Jakubowski, Edward M. ; Mcguire, Jeffery M. ; Evans, Ronald A. ; Hulet, Stanley W. ; Mioduszewski, Robert J. ; Thomson, Sandra A.
Author Organization(s):
EDGEWOOD CHEMICAL BIOLOGICAL CENTER ABERDEEN PROVING GROUND MD
Descriptive Note:
Conference paper
Supplementary Note:
Presented at the Army Science Conference (25th), "Transformational Army Science and Technology - Charting the future of S&T for the Soldier," held in Orlando, Florida on 27-30 Nov 2006. Published in proceedings of the same. See also ADM002075. The original document contains color images.
Pagination:
0009

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release
Distribution Statement:
Approved For Public Release; Distribution Is Unlimited.

RECORD

Collection: TR
Identifying Numbers
Monitor Series:
ECBC
 Subject Terms
Joint Capability Areas:
JCA_5_Command and Control; JCA_5.3_Planning; JCA_5.3.1_Analyze Problem; JCA_8_Building Partnerships
Communities of Interest:
No COI(s) Identified
Descriptor(s):
*GB AGENT, NEUROTRANSMITTERS, FLUORIDES, BLOOD CELLS, TOXICOLOGY, SWINE, MEDICAL RESEARCH, ACETYLCHOLINESTERASE
Field(s)/Group(s):
Biochemistry, Toxicology, Chemical, Biological and Radiological Warfare
Keyword(s):
*CYCLOSARIN, *FLUORIDE ION REGENERATION METHOD, *EXPOSURE ASSESSMENT, SARIN, BIOMARKERS, GOTTINGEN MINIPIGS, ISOTOPE DILUTION LVI(LARGE VOLUME INJECTOR) METHOD
Report Date:
2006 Nov 01
Creation Date:
2008 Jun 11