Regulation of DNA Metabolism by DNA-Binding Proteins Probed by Single Molecule Spectroscopy

reportActive / Technical Report | Accession Number: ADA459264 | Open PDF

Abstract:

Recent advances in single-molecule force spectroscopy of DNA make it possible to study the thermodynamics and kinetics of DNA binding proteins under a wide range of conditions. A biophysical model for the DNA binding T4 gene 32 protein has been developed to study the kinetics of DNA protein binding to transient single-stranded DNA regions due to thermal fluctuations. The model is used to analyze recent single-molecule spectroscopy data of this system.

Author(s):
Hanke, Andreas
Author Organization(s):
TEXAS UNIV AT BROWNSVILLE AND TEXAS SOUTHMOST COLLEGE
Descriptive Note:
Final technical rept., 1 Jun 2004-31 May 2006
Supplementary Note:
DOI: 10.21236/ADA459264
Pagination:
0008

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release
Distribution Statement:
Approved For Public Release; Distribution Is Unlimited.

RECORD

Collection: TR
Identifying Numbers
Report Number(s):
AFRL-SR-AR-TR-06-0488
Contract/Grant Number(s):
FA9550-04-1-0394
Monitor Series:
TR-06-0488, AFOSR
 Subject Terms
Joint Capability Areas:
JCA_3.2_Engagement; JCA_3_Force Application; JCA_3.2.1_Kinetic Means; JCA_8_Building Partnerships
Communities of Interest:
Energy and Power Technologies
Descriptor(s):
*METABOLISM, *DEOXYRIBONUCLEIC ACIDS, MOLECULAR SPECTROSCOPY, REGULATIONS, RECEPTOR SITES(PHYSIOLOGY), THERMODYNAMICS, PROTEINS
Field(s)/Group(s):
Biochemistry, Atomic and Molecular Physics and Spectroscopy
Report Date:
2006 Dec 05
Creation Date:
2007 Jan 10