EGFR Activation by Spatially Restricted Ligands

reportActive / Technical Report | Accession Number: ADA459169 | Open PDF

Abstract:

Misregulation of Transforming Growth Factor alpha TGFalpha and increased Epidermal Growth Factor Receptor Egfr activity has been associated with an increased prognosis of breast cancer. During cogenesis in Drosophila melanogaster local Egfr activation by the spatially-restricted TGFalpha-like ligand Gurken Grk is required for axis formation in the egg and future embryo. Squid Sqd a heterogeneous nuclear ribonucleoprotein hnRNF functions in the localization and translational regulation of grk mRNA. The purpose of this project is to identify factors that function with Sqd to produce spatially-restricted Egfr activation. We have integrated genetic and biochemical methods to identify and characterize the following factors that interact with Sqd 1 an arginine methyltransferase CG6554 which may serve to methylate Sqd in vivo, 2 Hrb27C an hnRNF protein and Ovarian tumor which are required for proper grk expression and 3 Bruno Cup and polyA-binding protein which function together to mediate grk translational regulation.

Author(s):
Clouse, Katherine N. ; Goodrich, Jennifer S.
Author Organization(s):
PRINCETON UNIV NJ
Descriptive Note:
Annual summary rept. 1 Jun 2003-31 May 2006
Supplementary Note:
The original document contains color images.
Pagination:
0052

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release
Distribution Statement:
Approved For Public Release; Distribution Is Unlimited.

RECORD

Collection: TR
Identifying Numbers
Contract/Grant Number(s):
DAMD17-03-1-0393
Monitor Series:
USAMRMC
 Subject Terms
Joint Capability Areas:
JCA_7.2.1_Mitigate Lethal Effects; JCA_7.2_Mitigate; JCA_7_Protection; JCA_5_Command and Control; JCA_5.3_Planning; JCA_1.2.7_Experimentation; JCA_1.3.2_Personnel Management; JCA_5.5.2_Task; JCA_5.5_Direct; JCA_1.2.3_Educating; JCA_1.2.5_Lessons Learned; JCA_JCA_4.1.2_Sustain the Force; JCA_4.1_Deployment and Distribution
Communities of Interest:
No COI(s) Identified
Descriptor(s):
*LIGANDS, *MOLECULAR BIOLOGY, *RIBONUCLEIC ACIDS, *DROSOPHILA, *OVARIAN CANCER, *BREAST CANCER, *GENETICS, ACTIVATION, PREDICTIONS, OVARIES, EPIDERMIS, EMBRYOS, IN VIVO ANALYSIS, BIOCHEMISTRY, PROTEINS
Field(s)/Group(s):
Genetic Engineering and Molecular Biology, Biology, Medicine and Medical Research, Organic Chemistry
Keyword(s):
*EPIDERMAL GROWTH FACTOR RECEPTOR, RNA LOCALIZATION, TRANSLATIONAL REGULATION, EGFR(EPIDERMAL GROWTH FACTOR RECEPTOR), HNRNP(HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN), METHYLTRANSFERASE, TRANSFORMING GROWTH FACTOR ALPHA
Report Date:
2006 Jun 01
Creation Date:
2007 Jan 10