Pulsed Electric Field Effects on Biological Cells

reportActive / Technical Report | Accession Number: ADA399182 | Open PDF

Abstract:

A new type of field-cell interaction, Intracellular Electromanipulation, by means of nonthermal, wideband electromagnetic radiation is being studied. It is based on capacitive coupling to cell substructures, and has therefore the potential to affect transport processes across subcellular membranes. This was verified experimentally by applying electrical pulses of 60 nanosecond duration, with electric field amplitudes of 50kVcm to human eosinophils in vitro. Besides causing poration of intracellular membranes without disrupting the outer cell membrane, sub-microsecond pulses were found to induce apoptosis in cells.

Author(s):
Schoenbach, Karl H. ; Beebe, Stephen J. ; Buescher, E. S. ; Liu, Shenggang
Author Organization(s):
OLD DOMINION UNIV RESEARCH FOUNDATION NORFOLK VA
Descriptive Note:
Final rept. 1 Dec 1998-31 Nov 2001
Supplementary Note:
DOI: 10.21236/ADA399182
Pagination:
0058

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release

RECORD

Collection: TR
Identifying Numbers
Report Number(s):
AFRL-SR-BL-TR-02-0040
Contract/Grant Number(s):
F49620-99-1-0069
Task Number(s):
EX
Project Number(s):
2301
Monitor Series:
TR-02-0040, AFOSR
 Subject Terms
Joint Capability Areas:
JCA_5.3_Planning; JCA_5_Command and Control; JCA_1.2.3_Educating; JCA_1.3.2_Personnel Management; JCA_1.2.7_Experimentation; JCA_JCA_4.1.2_Sustain the Force; JCA_4.1_Deployment and Distribution
Communities of Interest:
Biomedical
Descriptor(s):
*ELECTRIC FIELDS, *CELLS(BIOLOGY), COUPLING(INTERACTION), MEMBRANES(BIOLOGY), PULSES, ELECTROMAGNETIC RADIATION, BROADBAND, AMPLITUDE, EOSINOPHILS
Field(s)/Group(s):
Anatomy and Physiology, Medicine and Medical Research, Electricity and Magnetism
Keyword(s):
PE61102F, WUAFRL2301EX
Report Date:
2001 Nov 30
Creation Date:
2002 Mar 20