Guidance for Performance of the H4IIE Dioxin Screening Assay.

reportActive / Technical Report | Accession Number: ADA338374 | Open PDF

Abstract:

This technical note provides protocols for maintenance of cell cultures and for the conduct of a biomarker-based screening assay for dioxin toxic equivalents TCDD TEQs in sediments and other environmental samples using the H4IIE rat hepatoma cell line as performed at the U.S. Army Engineer Waterways Experiment Station WES. The purpose of these protocols is to provide analysts who may wish to gain the capability for performing the assay with a detailed blueprint of how the assay is conducted and of what is necessary in terms of technique, materials, instrumentation, and time.

Author(s):
No Author(s) Identified
Author Organization(s):
ARMY ENGINEER WATERWAYS EXPERIMENT STATION VICKSBURG MS
Pagination:
0024

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release

RECORD

Collection: TR
Identifying Numbers
Report Number(s):
DOER-C1
Monitor Series:
AEWES
 Subject Terms
Joint Capability Areas:
JCA_8_Building Partnerships; JCA_8.1.1_Inform Domestic and Foreign Audiences; JCA_8.1_Communicate; JCA_6_Net Centric; JCA_6.1.3_Switching and Routing; JCA_6.1_Information Transport; JCA_5.3_Planning; JCA_5_Command and Control; JCA_4.5.4_Requirements Definition; JCA_4.5_Operational Contract Support; JCA_3.2_Engagement; JCA_3_Force Application; JCA_4.3_Maintain; JCA_8.2_Shape
Communities of Interest:
No COI(s) Identified
Descriptor(s):
*CELLS(BIOLOGY), *CULTURES(BIOLOGY), *TOXIC DISEASES, *DIOXINS, MAINTENANCE, ENVIRONMENTS, SAMPLING, SEDIMENTS
Field(s)/Group(s):
Medicine and Medical Research, Toxicology
Keyword(s):
HEPATOMA CELLS, H4IIE CELLS
Report Date:
1998 Feb 01
Creation Date:
1998 Mar 25