Regulation of Anchorage-Independent Growth in Breast Cancer: Role of Signalling by Extracellular Matrix and Growth Factors

reportActive / Technical Report | Accession Number: ADA331130 | Open PDF

Abstract:

In this report we investigate the role of ECM proteins and growth factors in anchorage-independent survival and growth of a murine mammary carcinoma-derived cell line, designated SP1. We demonstrate that SP1 cells adhere well to FN and VN and express a number of integrins, including a5beta1, alpha v beta 3, alpha 6 beta 1, alpha 3 beta 1 and alpha 2 beta 1, the latter being upregulated under anchorage- independent conditions. We also demonstrated by partially removing FN from 7 FBS or adding FN to 1 FBS that FN is required for SP1 cell colony growth.

Author(s):
Saulnier, Ron
Author Organization(s):
QUEEN'S UNIV KINGSTON (ONTARIO)
Descriptive Note:
Final rept. 1 Aug 94-29 Jul 97
Pagination:
0319

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release

RECORD

Collection: TR
Identifying Numbers
Contract/Grant Number(s):
DAMD17-94-J-4127
Monitor Series:
USAMRMC
 Subject Terms
Joint Capability Areas:
JCA_7.2.1_Mitigate Lethal Effects; JCA_7.2_Mitigate; JCA_7_Protection; JCA_5_Command and Control; JCA_1.2.7_Experimentation; JCA_5.3_Planning; JCA_1.3.2_Personnel Management; JCA_1.2.5_Lessons Learned; JCA_5.6.3_Assess Achievement of Objectives; JCA_5.6_Monitor; JCA_1.2.3_Educating; JCA_4.3_Maintain; JCA_5.2_Understand; JCA_1.2.6_Concepts; JCA_5.2.2_Develop Knowledge and Situational Awareness
Communities of Interest:
No COI(s) Identified
Descriptor(s):
*GROWTH(PHYSIOLOGY), *BREAST CANCER, PROTEINS, MUTATIONS, CLONES, TRANSDUCERS, CELLS(BIOLOGY), COLONIES(BIOLOGY), MAMMARY GLANDS, TYROSINE, PHOSPHORYLATION, PHOSPHORUS TRANSFERASES, METASTASIS
Field(s)/Group(s):
Biochemistry, Genetic Engineering and Molecular Biology, Medicine and Medical Research
Keyword(s):
INTEGRINS, ECM(EXTRACELLULAR MATRIX), SIGNAL TRAANSDUCTION
Report Date:
1997 Jul 01
Creation Date:
1997 Nov 12