Human Mammary Cell Resource.

My laboratory has generated a human mammary epithelial cell HMEC system for studies on normal and abeirant growth control mechanisms. As part of the goal to facilitate widespread use of HMEC, we have provided cells and cell culture expertise to over 150 laboratories worldwide. To optimize use of this resource, we developed a complex database for storage and retrieval of information on the HMEC, and newsletters for communication among those using these HMEC. The purpose of this grant is to 1 upgrade and expand the features of this database, 2 ensure that all computer and written records can be readily understandable to others besides the PI, 3 create written histories of complicated cell lineages and enter them into the database, 4 develop an Email bulletin board to foster rapid, cooperative communication among laboratories using HMEC. Most work on the database upgrade was completed in Year 1. Only minor additional changes have been necessary. For year 2, old records were entered into the database and work was begun on compiling cell histories and making written records more accessible. A world wide web has been launched. This work is proceeding as planned, although it is clear that the history compilation will require much time.