Selective Protein Toxin Destruction Using Hydrolyzing Catalytic Monoclonal Antibodies.

reportActive / Technical Report | Accession Number: ADA312073 | Open PDF

Abstract:

The problem of amide hydrolysis using catalytic antibodies was analyzed and attacked. A novel, direct screening for catalysis method was developed which allows detection of such antibodies in hybridoma supernatants. The method catELISA involves a solid-phase substrate and an anti-product ELISA assy it was found to be general and highly effective using ester-hydrolyzing anti-phosphonate hapten antibodies. By employing autoimmune-prone mouse strains, vastly increased numbers of catalytic antibodies for ester hydrolysis were produced. All attempts using catELISA and these exotic mouse strains to obtain amide- or peptide-hydrolyzing antibodies by immunization with simple phosphonate haptens as transition state analogs failed. Therefore, the synthesis of new, more sophisticated transition state analogs as haptens was carried out. 2-Aminoalcohols, aminophosphinates, cyclic phosphinates and phosphonimides were pursued haptens for hydrolysis of acyl-proline, phenylalanyl amides were prepared. The many hybridomas studied using these haptens failed to produce arnide-hydrolyzing catalysts. Our results emphasize the need for additional approaches to attack this challenging problem. However, the results do show that powerful antibodies can be produced, at least for ester bonds, and they have made available new technologies that may eventually afford sequence-specific catalytic antibodies.

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