X-Ray Crystallographic Studies on Acetylcholinesterase and on its Interaction with Anticholinesterase Agents.

reportActive / Technical Report | Accession Number: ADA307114 | Open PDF

Abstract:

The objective of this project was to solve, for the first time, by X-ray crystallography, the three-dimensional structure of acetylcholinesterase AChE and to learn from this structure the reaction mechanism, inhibition modes and other structural and functional properties of the enzyme. The principal objectives of the current project included 1 Attempts to obtain crystals of cholinesterases other than T. californica AChE, in particular of AChE of human origin. 2 Collection of improved data sets, viz. at higher resolution than previously, for crystals of Torpedo AChE. 3 Determination of complexes of Torpedo AChE with various ligands, including orgamophosphates. In the this project, we have made progress towards all three objectives, as well as in additional areas related to the long-term aims of our research program

Author(s):
Silman, Israel ; Sussman, Joel
Author Organization(s):
WEIZMANN INST OF SCIENCE REHOVOT (ISRAEL)
Descriptive Note:
Final rept. 30 Apr 93-30 Sep 95,
Pagination:
0073

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release, Document Partially Illegible
Distribution Statement:
Availability: Document Partially Illegible.

RECORD

Collection: TR
Identifying Numbers
Contract/Grant Number(s):
DAMD17-93-C-3070
Monitor Series:
USAMRMC
 Subject Terms
Joint Capability Areas:
JCA_5_Command and Control; JCA_5.3_Planning; JCA_5.3.4_Develop Courses of Action; JCA_5.3.5_Analyze Courses of Action; JCA_5.3.3_Develop Strategy; JCA_1.2.7_Experimentation
Communities of Interest:
Weapons Technologies
Descriptor(s):
*CRYSTALS, *X RAYS, *ACETYLCHOLINESTERASE, *CHOLINESTERASE, *CRYSTALLOGRAPHY, DATA BASES, TORPEDOES, HUMANS, STRUCTURAL PROPERTIES, ENZYMES, STRUCTURES, HIGH RESOLUTION, THREE DIMENSIONAL, RESPONSE, LIGANDS, FUNCTIONAL ANALYSIS, INHIBITION, CHOLINESTERASE INHIBITORS
Field(s)/Group(s):
Biochemistry, Radiobiology, Crystallography
Keyword(s):
TORPEDO CALIFORNICA, ANTICHOLINESTERASE AGENTS
Report Date:
1995 Dec 01