Amplified Genes in Breast Cancer: Molecular Targets for Investigation and Therapy.

reportActive / Technical Report | Accession Number: ADA300593 | Open PDF

Abstract:

The primary objective has been development of a rapid method for purifying extrachromosomally amplified genes DMs from tumor cell lines without relying on cytogenetics. DMs were purified by isolating micronuclei induced by treatment with hydroxyurea. Micronuclei were dissociated from nuclei by lysis under conditions that disaggregated intermediate filaments. Rate zonal and density centrifugation steps completely separated micronuclei from nuclei, enabling preparation of probes for fluorescence in situ hybridization to metaphase chromosomes of normal peripheral blood lymphocytes to identify the genomic regions from which the DMs were generated. Four cell lines derived from different types of tumors with DM contents spanning the range typically reported in human biopsy material e.g., 2-50 per nucleus were analyzed.

Author(s):
Wahl, Geoffrey M.
Author Organization(s):
SALK INST FOR BIOLOGICAL STUDIES LA JOLLA CA
Descriptive Note:
Annual rept. 1 Sep 94-31 Aug 95,
Pagination:
0043

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release

RECORD

Collection: TR
Identifying Numbers
Contract/Grant Number(s):
DAMD17-94-J-4359
Monitor Series:
USAMRMC
 Subject Terms
Joint Capability Areas:
JCA_5_Command and Control; JCA_5.3_Planning; JCA_5.3.3_Develop Strategy; JCA_5.6.4_Assess Guidance; JCA_5.6_Monitor; JCA_1.2.7_Experimentation; JCA_1.2.3_Educating; JCA_1.2.5_Lessons Learned; JCA_1.3.2_Personnel Management
Communities of Interest:
No COI(s) Identified
Descriptor(s):
*CHROMOSOMES, *MAMMARY GLANDS, *BREAST CANCER, FUNCTIONS, DENSITY, PROBES, FLUORESCENCE, HUMANS, MOLECULES, MATERIALS, TARGETS, SENSITIVITY, PURIFICATION, FILAMENTS, GENES, GENETICS, LYMPHOCYTES, BLOOD, NUCLEI, CENTRIFUGE SEPARATION, BIOPSY, CYTOLOGY, CHEMOTHERAPEUTIC AGENTS, HYBRIDIZATION, LYSIS
Field(s)/Group(s):
Biology, Medicine and Medical Research
Keyword(s):
MICRONUCLEUS PURIFICATION, GENE AMPLIFICATION, DOUBLE MINUTE CHROMOSOME PURIFICATION, HYDROXYUREA
Report Date:
1995 Sep 01