Evaluation of the Metabolic Fate of the Munition Material (TNT and RDX) in Plant Systems and Initial Assessment of Material Interaction with Plant Genetic Material (DNA) - Initial Assessment of Plant DNA Adducts as Biomarkers.

reportActive / Technical Report | Accession Number: ADA298520 | Open PDF

Abstract:

This exploratory study performs preliminary ground work towards identifying adducts formed between DNA and metabolically activated trinitrotoluene TNT intermediates for potential assessment of their applicability as biomarkers of environmental contamination. Chromatographic comparisons between control and TNT-adducted hydrolysates allowed the tentative identification of two TNT-adducted nucleotides. Chromatographic profiles of TNT-adducted DNA hydrolysates featured these compounds eluting in the 21.4- to 22.6-min retention window. The suspect adducts were not observed in all TNT adduct reactions performed, perhaps because of the formation at concentrations below the analytical detection limit. These studies can be added to the mounting evidence that specific TNT-DNA adducts may occur but definative proof was not obtained.

Author(s):
Harvey, Scott D. ; Clauss, Therese W. ; Fellows, Robert J. ; Cataldo, Dominic A.
Author Organization(s):
DEPARTMENT OF ENERGY RICHLAND WA RICHLAND OPERATIONS OFFICE
Descriptive Note:
Final rept.,
Pagination:
0034

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release

RECORD

Collection: TR
Identifying Numbers
Monitor Series:
USAMRMC
 Subject Terms
Joint Capability Areas:
JCA_5_Command and Control; JCA_5.3_Planning; JCA_5.3.3_Develop Strategy; JCA_5.3.4_Develop Courses of Action; JCA_5.3.5_Analyze Courses of Action; JCA_1.3.2_Personnel Management; JCA_4.3_Maintain; JCA_1.2.5_Lessons Learned
Communities of Interest:
No COI(s) Identified
Descriptor(s):
*DEOXYRIBONUCLEIC ACIDS, *RDX, *TNT, *PLANTS(BOTANY), ACTIVATION, DETECTION, INTERACTIONS, PROFILES, AMMUNITION, CONTAMINATION, TRACER STUDIES, CHROMATOGRAPHS
Field(s)/Group(s):
Biochemistry, Ammunition and Explosives, Medicine and Medical Research
Report Date:
1995 Jun 01