Enzymatic Hydrolysis and Solvent Extraction Method of Fat Determination

Fats are recognised as important dietary components, with water, protein and carbohydrate. Fat has particular significance because it yields twice the energy compared with the other macro-nutrients. Military rations are designed to provide military personnel with adequate nutrition in as compact and light weight manner as possible. Military activities require high levels of energy expenditure. Current nutritional wisdom is that energy derived from fat should not exceed 33 of total energy intake 1. Furthermore, polyunsaturated fatty acids are essential in animal nutrition, particularly human nutrition with a recommended intake of 1.5 to 8 of energy 2. Thus, there is need for a consistent method for determination of fat in foods.