Biofilm Ecology of Bioluminescent Bacteria

Test systems have been developed which enable the evaluation of bacterial biofilm formation and metabolic activity under conditions simulating those of the in situ environment. A series of laminar-flow adhesion cells were constructed with provisions for on-line, non-destructive measurements of bioluminescence, fluorescence, open circuit potential, and po2 for monitoring colonization and succession as influenced by a systematic change in bulk-phase and substratum conditions. Bioluminescence and fluorescence by biofilms of the bioluminescent, marine bacterium, Vibrio harveyi were utilized as endpoints for adhesion in evaluations of antifouling AF ship hull coatings. Resistance to colonization of V. harveyi was noted in the order of F-121 Navy BRA 640 IP 15 DNP. Future work will evaluate changes in cellular lipid biomarkers associated with biofilms and planktonic cultures exposed to copper contained in AF coatings and in bulk-phase chemostats, respectively. The test systems and procedures developed under ONR funding will enable studies of materials compatibility, antifouling efficacy, and biocide toxicity in diverse ecosystems. Bioluminescent bacteria, lux, alg, biofilm, antifouling agents, biocide efficacy, biofilm ecology, flow cells, fluorometry, ship hull coatings, Vibrio harveyi.