Analysis of Saxitoxin from Urine Using Dynamic FAB/MS

reportActive / Technical Report | Accession Number: ADA244960 | Open PDF

Abstract:

An improved method of saxitoxin analysis in urine using continuous Flow Fast Atom Bombardment Mass Spectrometry CFFABMS was developed. Parameters studied were matrix composition, matrix flow rate, temperature of probe tip, probe tip design and sample extraction. Optimal detection was obtained using the following matrix composition 5 glycerol, 0.5 acetic acid, 0.025 sodium dodecylsulfate, 0.1 polyethylene glycol PEG 400 and 0.5 PEG 300 probe tip temperature -55 C flow rate 5 or 8 microliter per min probe tip Olson-Hogge design. The STX standard was detected at 200 picograms with signal-to-noise ratio of 11. The percent recovery of saxitoxin from human urine after cleanup on a weak cation exchange CBA Bond-Elut Analytichem Inter. Varian, Anaheim, CA column was 75.

Author(s):
Mirocha, Chester J. ; Kim, Young B. ; Mirza, Urooj ; Xie, Weiping ; Abbas, Hamed K.
Author Organization(s):
MINNESOTA UNIV ST PAUL DEPT OF PLANT PATHOLOGY
Descriptive Note:
Final rept. 15 Jul 1989-14 Aug 1991,
Supplementary Note:
DOI: 10.21236/ADA244960
Pagination:
0032

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release
Distribution Statement:
Approved For Public Release; Distribution Is Unlimited.

RECORD

Collection: TR
Identifying Numbers
Contract/Grant Number(s):
DAMD17-89-C-9101
Task Number(s):
AH
Project Number(s):
3M263002D807
Monitor Series:
USAMRDC
 Subject Terms
Joint Capability Areas:
JCA_5_Command and Control; JCA_5.3_Planning; JCA_7.2.1_Mitigate Lethal Effects; JCA_7.2_Mitigate; JCA_7_Protection; JCA_1.2.7_Experimentation; JCA_1.2.3_Educating
Modernization Areas:
Microelectronics
Communities of Interest:
Biomedical
Descriptor(s):
*CATIONS, DETECTION, HUMANS, ION EXCHANGE, FLOW RATE, COMPOSITION(PROPERTY), LOW STRENGTH, URINE, OPTIMIZATION, MATRIX MATERIALS
Field(s)/Group(s):
Biochemistry
Keyword(s):
*CHEMICAL ANALYSIS, *URINE, AS807, MASS SPECTROMETRY, CHEMICAL COMPOSITION, SAMPLE EXTRACTION, PROBE TIP DESIGN, TEMPERATURE MEASURING INSTRUMENTS, SAXITOXIN ANALYSIS, PE63002A, WUDA318543
Report Date:
1991 Oct 21