Glutamate/NMDA Receptor Ion-Channel Purification, Molecular Studies and Reconstitution into Stable Matrices

reportActive / Technical Report | Accession Number: ADA244727 | Open PDF

Abstract:

This is the final report of a project that had as its primary focus the isolation of the NMDA receptor protein complex and the reconstitution of these proteins for the development of biosensors. The progress made in all phases of this work has met the goals selected for the research. 1 Two proteins of the receptor were fully purified and characterized. 2 The intact complex was purified and biochemically characterized. 3 One of the protein CDNAs was cloned and sequenced. 4 The complex was reconstituted into liposomes and planar lipid bilayers. 5 A prototype sensor based on this receptor complex was developed. 6 Cell model systems for the study of the receptor were developed.

Author(s):
Michaelis, Elias K.
Author Organization(s):
KANSAS UNIV LAWRENCE CENTER FOR BIOMEDICAL RESEARCH
Descriptive Note:
Final rept. 15 Mar 1988-1 Jul 1991
Pagination:
0006

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release
Distribution Statement:
Approved For Public Release; Distribution Is Unlimited.

RECORD

Collection: TR
Identifying Numbers
Report Number(s):
ARO-25702.1-LS
Contract/Grant Number(s):
DAAL03-88-K-0017
Monitor Series:
25702.1-LS, ARO
 Subject Terms
Communities of Interest:
Biomedical
Descriptor(s):
*DETECTORS, BIOLOGICAL DETECTION, CELLS(BIOLOGY), CLONES, GLUTAMIC ACID, ISOLATION, LAYERS, LIPIDS, LIPOSOMES, MODELS, MOLECULES, PLANAR STRUCTURES, PROTEINS, PROTOTYPES, SALTS, SENSE ORGANS
Field(s)/Group(s):
Medicine and Medical Research
Keyword(s):
*BIOSENSORS, DIOXYRIBONUCLEIC ACID
Report Date:
1991 Aug 29
Creation Date:
1992 Feb 18