Effects of Clindamycin on Adherence of Clostridium difficile to Human Embryonic Intestinal Cells

reportActive / Technical Report | Accession Number: ADA244262 | Open PDF

Abstract:

An in vitro assay system, consisting of monolayers of human embryonic intestinal cells HEI and Clostridium difficile, was used to observe cell surface and cytoplasmic interactions. Microorganism test conditions include toxin B positive 938 and toxin B negative 789 strains, each harvested from standard sheep blood agar SBA, casamino acids agar CAS, and CAS containing subinhibitory concentrations of clindamycin CAS-CL. Effects of toxin B were seen when organisms were harvested from SBA. Adherence and internalization were observed with organisms harvested from CAS and CAS-CL. Evidence of adherence andor movement into HEI cells was observed at 15, 30, 60, and 90 min time periods using the in vitro internalization assay, conventional scanning electron microscopy, transmission electron microscopy, and field emission scanning electron microscopy of freeze fractured preparations. Quantitative organism determinations, and the observed activity of microorganisms harvested from CAS and CAS-CL, revealed their capability to adhere to andor enter HEI cells. The toxin B producer, 938, harvested from CAS-CL, entered the cytoplasm and remained intact within it.

Author(s):
Wood, Sheila J. ; Dalton, H. P. ; Mumaw, V.
Author Organization(s):
CHEMICAL RESEARCH DEVELOPMENT AND ENGINEERING CENTER ABERDEEN PROVING GROUND MD
Descriptive Note:
Final rept.,
Supplementary Note:
Prepared in collaboration with Medical Coll. of Virginia, Richmond.
Pagination:
0036

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release
Distribution Statement:
Approved For Public Release; Distribution Is Unlimited.

RECORD

Collection: TR
Identifying Numbers
Report Number(s):
CRDEC-TR-311
Monitor Series:
CRDEC
 Subject Terms
Joint Capability Areas:
JCA_1.2.3_Educating
Communities of Interest:
Biomedical
Descriptor(s):
*SCANNING ELECTRON MICROSCOPES, HUMANS, INTERACTIONS, CLOSTRIDIUM, FRACTURE(MECHANICS), IN VITRO ANALYSIS, ELECTRON MICROSCOPY, TRANSMITTANCE, SURFACES, FREEZING, CONCENTRATION(COMPOSITION), CYTOPLASM, EMBRYOS, CELLS(BIOLOGY), ASSAYING, INTESTINES, TOXINS AND ANTITOXINS, BLOOD, ANTIBIOTICS, MICROORGANISMS, SHEEP, AGAR, PREPARATION
Field(s)/Group(s):
Biochemistry
Keyword(s):
*CLINDAMYCIN, *CYTOPATHIC, *CYTOTOXIGENIC, *CASAMINO ACIDS MEDIA, ADHERENCE, FREEZE FRACTURE, HUMAN EMBRYONIC INTESTICAL CELLS, SCANNING ELECTRON MICROSCOPY, TRANSMISSION ELECTRON MICROSCOPY
Report Date:
1991 Nov 01