Further Development and Validation of the frog Embryo Teratogenesis Assay - Xenopus (FETAX)

reportActive / Technical Report | Accession Number: ADA236986 | Open PDF

Abstract:

The goal of the project was to develop and validate the Frog Embryo Teratogenesis Assay- Xenopus FETAX. FETAX is a 96 h whole embryo bioassay designed to rapidly assess the developmental toxicity of pure compounds and complex mixtures. The five specific objectives of this study are 1 validate FETAX using direct-acting compounds of known mammalian developmental toxicity, 2 develop and validate an in vitro metabolic activation system using rat liver microsomes, 3 explore and use three carrier solvents which will help solubilize non-polar compounds, 4 develop an Atlas of Abnormalities as a companion manual to the ASTM New Standard Guide for the Conduct of FETAX and, 5 compare the sensitivity of FETAX with a fathead minnow bioassay modified to have the same exposure conditions as FETAX.

Author(s):
Bantle, John A.
Author Organization(s):
OKLAHOMA STATE UNIV STILLWATER
Descriptive Note:
Final rept. 26 Sep 1988-31 Dec 1990
Pagination:
0556

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release
Distribution Statement:
Approved For Public Release; Distribution Is Unlimited.

RECORD

Collection: TR
Identifying Numbers
Contract/Grant Number(s):
DAMD17-88-C-8031
Task Number(s):
00
Project Number(s):
3E162720A835
Monitor Series:
USAMRDC
 Subject Terms
Joint Capability Areas:
JCA_7.2.1_Mitigate Lethal Effects; JCA_7.2_Mitigate; JCA_7_Protection; JCA_5_Command and Control; JCA_5.3_Planning; JCA_5.3.4_Develop Courses of Action; JCA_5.3.5_Analyze Courses of Action; JCA_5.3.3_Develop Strategy; JCA_1.2.7_Experimentation; JCA_1.2.3_Educating; JCA_1.2.5_Lessons Learned; JCA_4.2_Supply; JCA_4.2.2_Inventory Management; JCA_4.5.4_Requirements Definition; JCA_4.5_Operational Contract Support
Modernization Areas:
Fully Networked C3
Communities of Interest:
No COI(s) Identified
Descriptor(s):
*METABOLISM, *IN VITRO ANALYSIS, EXPOSURE(GENERAL), RATS, TOXICITY, MIXTURES, PURITY, SOLVENTS, LIVER, TERATOGENIC COMPOUNDS, EMBRYOS, ASSAYING, FROGS, ABNORMALITIES, MICROSOMES, BIOASSAY, MINNOWS, ACTIVATION, VALIDATION
Field(s)/Group(s):
Medicine and Medical Research
Keyword(s):
FROG EMBRYO TERATOGENESIS ASSAY XENOPUS (FETAX) DEVELOPMENTAL TOXICOLOGY, TERATOLOGY, *VALIDATION METABOLIC ACTIVATION SYSTEM, CYTOCHROME P 450, RAIII, PE62720A
Report Date:
1991 Feb 28