Debaryomyces hansenii: A Model System for Marine Molecular Biology

reportActive / Technical Report | Accession Number: ADA236966 | Open PDF

Abstract:

In an attempt to construct plasmids with the capability of autonomous replication we have isolated two ARS from Debaryomyces utilizing S. cerevisiae strain SEY 2108 Ura3 and its corresponding gene URA3 as a marker. Attempts are now being made to transform Ura3 Debaryomyces with this plasmid. Transformation attempted with techniques such as protoplasting, alkali cations and electroporation has not met with success. With the arrival of strain J-26 for Dr. L. Adler, transformation will be attempted utilizing hygromycin resistance as a marker. Dr. Adlers has also agreed to generate Ura3 mutants of his strain for use as a biological marker. To generate auxotrophic mutations for transformation, mutagenesis was performed utilizing Ethyl Methyl Sulfonate.

Author(s):
Nadathur, Govind S.
Author Organization(s):
CALIFORNIA UNIV SANTA BARBARA
Descriptive Note:
Annual progress rept. 1 Jun 1990-30 May 1991,
Supplementary Note:
DOI: 10.21236/ADA236966
Pagination:
0004

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release
Distribution Statement:
Approved For Public Release; Distribution Is Unlimited.

RECORD

Collection: TR
Identifying Numbers
Contract/Grant Number(s):
N00014-89-J-3027
Monitor Series:
ONR
 Subject Terms
Joint Capability Areas:
JCA_5_Command and Control; JCA_5.3_Planning
Modernization Areas:
Autonomy
Communities of Interest:
Autonomy
Descriptor(s):
*CATIONS, METHYL RADICALS, MUTATIONS, ALKALI METAL COMPOUNDS, MARINE BIOLOGY, SULFONATES, MOLECULAR BIOLOGY, ETHYL RADICALS, MODELS, PLASMIDS
Field(s)/Group(s):
Biochemistry
Report Date:
1991 May 30