Genetic Engineering of Clostridium Difficile Toxin a Vaccine

reportActive / Technical Report | Accession Number: ADA230411 | Open PDF

Abstract:

The long range goal of this project is to use C. difficile toxin A as a model to add technical information in the area of vaccine biotechnology using recombinant DNA techniques. This will be accomplished in part by predicting epitopes from the DNA sequence of toxin A, synthesizing DNA for these predicted regions, and testing their antigenic activity in expression vectors. Part of the toxin A gene has been cloned and sequenced. A total of twelve different epitodes have been predicted, synthesized, cloned into lambda gtll and tested for antigenicity. Alternative procedures for epitode mapping has been described and initiated.

Author(s):
Muldrow, Lycurgus L. ; Johnson, Joe
Author Organization(s):
SPELMAN COLL ATLANTA GA
Descriptive Note:
Annual rept. 15 Jun 1989-14 Jun 1990
Supplementary Note:
DOI: 10.21236/ADA230411
Pagination:
0016

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release, Document Partially Illegible
Distribution Statement:
Availability: Document Partially Illegible.

RECORD

Collection: TR
Identifying Numbers
Contract/Grant Number(s):
DAMD17-87-C-7135
Task Number(s):
AA
Project Number(s):
3M162770A871
Monitor Series:
USAMRDC
 Subject Terms
Joint Capability Areas:
JCA_5_Command and Control; JCA_5.3_Planning; JCA_5.3.3_Develop Strategy; JCA_5.3.4_Develop Courses of Action; JCA_5.3.5_Analyze Courses of Action
Modernization Areas:
Biotechnology
Communities of Interest:
Biomedical
Descriptor(s):
*CLOSTRIDIUM, *GENETIC ENGINEERING, *VACCINES, SYNTHESIS, LONG RANGE(TIME), DEOXYRIBONUCLEIC ACIDS, SEQUENCES, LONG RANGE(DISTANCE), CLONES, BIOTECHNOLOGY, ANTIGENS, TOXINS AND ANTITOXINS
Field(s)/Group(s):
Medicine and Medical Research
Keyword(s):
CLOSTRIDIUM DIFFICILE, VACCINE, RECOMBINANT DNA, PE62770A, WU348, AS871
Report Date:
1990 Aug 16