IGG Subclass and Isotype Specific Immunoglobulin Responses to Lassa Fever and Venezuelan Equine Encephalomyelitis: Natural Infection and Immunization

Venezuelan equine encephalitis VEE specific immunoglobulin responses to the two vaccines, TC-83 a live attenuated vaccine and C-84 a formalin inactivated vaccine derived from the TC-83 strain were evaluated using an antigen and isotype specific enzyme-linked immunoadsorbent assay ELISA. As previously described, the assays were standardized in methodology and in relation to a uniform reference curve prepared from a pool of subjects with high titers of 80 PR-VNA 80 plaque reduction in the viral neutralization assay. Time-course SERA before and following varying schedules of TC-83 andor C-84 vaccine were tested suggesting a trend of improved responses to live attentuated vaccine particularly with IgA and IgM compared to the killed vaccine. Subject Terms Venezuela equine encephalitis immunoglobulin.