Secretory Polypeptides Encoded in Balbiani Ring Genes

reportActive / Technical Report | Accession Number: ADA220589 | Open PDF

Abstract:

The goal of this project is to learn the structure and assembly characteristics of a family of secretory proteins SPs. SPs are synthesized in salivary glands of aquatic larvae of the midge, Chironomus. Larvae construct underwater tubes for filter feeding and pupation using silk fibers composed of SPs. During the past year we made progress in two areas. 1 A 1-kb cDNA clone was sequenced that represents a portion of a 4.8-kb polyA RNA. Though it lacks sequences found in other SP mRNAs, its abundance in salivary glands is equal to other SP mRNAs. Antipeptide antibodies are being raised to identify the protein encoded by this mRNA. 2 Infrared and circular dichroism spectrometry have been used to study the structure of synthetic peptides representing the alternating constant and subrepeat domains of SPs. These 1000-kDal proteins appear to consist of alternating domains of alpha- and POLYGlyII-helices. KT

Author(s):
Case, Steven T.
Author Organization(s):
MISSISSIPPI UNIV MEDICAL CENTER JACKSON DEPT OF BIOCHEMISTRY
Descriptive Note:
Annual rept. 15 Apr 1989-14 Apr 1990,
Pagination:
0018

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release
Distribution Statement:
Approved For Public Release; Distribution Is Unlimited.

RECORD

Collection: TR
Identifying Numbers
Report Number(s):
7H432
Contract/Grant Number(s):
N00014-87-K-0387
Task Number(s):
RR0416441
Project Number(s):
RR0416
Monitor Series:
ONR
 Subject Terms
Joint Capability Areas:
JCA_1.2.3_Educating; JCA_4.6_Engineering; JCA_4.4.1_Food Service; JCA_4.4_Logistics Services; JCA_5_Command and Control
Communities of Interest:
No COI(s) Identified
Descriptor(s):
*PEPTIDES, *SECRETION, *POLYMERS, *GENES, *MOLECULAR STRUCTURE, IN VITRO ANALYSIS, SEQUENCES, ANTIBODIES, FILTERS, ASSEMBLY, RINGS, FEEDING, LARVAE, CIRCULAR, DICHROISM, AMINO ACIDS, SILK, DISASSEMBLY, SPECTROMETRY, PROTEINS, WATER, SALIVARY GLANDS, FIBERS, INTERACTIONS
Field(s)/Group(s):
Biochemistry, Genetic Engineering and Molecular Biology
Keyword(s):
*POLYPEPTIDES, *SECRETORY PROTEINS, *SECRETORY POLYPEPTIDES, BALBIANI RING GENES, POLYPEPTIDE FIBERS, PE61153N, WU028
Report Date:
1990 Apr 15