Crystallization of the Fab Fragment of a Monoclonal Anti-Spin-Label Antibody With and Without Bound Hapten

reportActive / Technical Report | Accession Number: ADA219761 | Open PDF

Abstract:

The structural change that an antibody undergoes upon binding of antigen is interesting in terms of antibody diversity and function. Independent crystal structures of an antibody both with and without antigen have not yet been solved. We report here the crystallization of the Fab fragment of AN02, a monoclonal antibody specific for a spin-labelled dinitrophenyl hapten, with and without hapten for the chemical structure of this hapten see Anglister et al. KT

Author(s):
Leahy, Daniel J. ; Hynes, Thomas ; Fox, Robert O. ; Mcconnell, Harden M.
Author Organization(s):
STANFORD UNIV CA STAUFFER LAB FOR PHYSICAL CHEMISTRY
Supplementary Note:
DOI: 10.21236/ADA219761
Pagination:
0005

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release
Distribution Statement:
Approved For Public Release; Distribution Is Unlimited.

RECORD

Collection: TR
Identifying Numbers
Contract/Grant Number(s):
N00014-86-K-0388
Monitor Series:
ONR
 Subject Terms
Joint Capability Areas:
JCA_1_Force Support; JCA_6.3.3_Spectrum Management; JCA_1.2_Force Preparation
Communities of Interest:
No COI(s) Identified
Descriptor(s):
*CRYSTALLIZATION, *MONOCLONAL ANTIBODIES, *MOLECULAR STRUCTURE, STRUCTURAL PROPERTIES, SITES, ANTIGENS, CLONES, MOLECULE MOLECULE INTERACTIONS, BINDERS, MOLECULES, MODIFICATION, CRYSTAL STRUCTURE, ANTIBODIES
Field(s)/Group(s):
Genetic Engineering and Molecular Biology
Keyword(s):
*ACTIVE SITES, BINDING SITES, HAPTENE, FAB FRAGMENTS
Report Date:
1987 Jan 01