DURIP - Upgrade of the Meridian ACAS-470 for Toxicological Research

reportActive / Technical Report | Accession Number: ADA217683 | Open PDF

Abstract:

This proposal was to have our current Meridian ACAS 570 laser cytometer upgraded with a 5 watt coherent laser, Olympus microscope with a CCD camera and video monitor, a mass data storage computer and dual detector system. This upgrade allowed us new dimensions of research potentials for studying mechanisms of chemical-induced toxicities in living cells, specifically enabling us to detect if chemicals caused toxicities by altering free Ca level, causing pH changes or induction of free radicals in single cells. During this year, we have pioneered the application of this new instrument in the demonstration that a heptachlor, a toxic pesticideneurotoxicant, caused increased of free Ca b several cytotoxic chemicals could generate free radicals and c oncogene products could be detected using fluorescent antibodies and d UV-induced DNA damage could be quantified using fluorescent antibodies. Keywords Laboratory equipment Meridian ACAS-570 Laser image analyzer Quantitative fluorescence detection Mechanisms of chemical toxicities.

Author(s):
Trosko, James E.
Author Organization(s):
MICHIGAN STATE UNIV EAST LANSING DEPT OF PEDIATRICS/HUMAN DEVELOPMENT
Descriptive Note:
Final rept. 1 Dec 1988-30 Nov 1989
Supplementary Note:
Original contains color plates: All DTIC/NTIS reproductions will be in black and white.
Pagination:
0084

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release

RECORD

Collection: TR
Identifying Numbers
Report Number(s):
AFOSR-TR-90-0115
Contract/Grant Number(s):
AFOSR-89-0114
Task Number(s):
A4
Project Number(s):
3842
Monitor Series:
TR-90-0115, AFOSR
 Subject Terms
Joint Capability Areas:
JCA_1.2.3_Educating; JCA_5_Command and Control; JCA_5.3_Planning; JCA_4.3_Maintain; JCA_4.5.4_Requirements Definition; JCA_4.5_Operational Contract Support; JCA_1.2.7_Experimentation; JCA_1.3.2_Personnel Management; JCA_JCA_4.1.2_Sustain the Force; JCA_4.1_Deployment and Distribution; JCA_5.2.3_Share Knowledge and Situational Awareness; JCA_5.2_Understand
Communities of Interest:
Biomedical
Descriptor(s):
*OPTICAL EQUIPMENT, *LABORATORY EQUIPMENT, *TOXICOLOGY, CALCIUM, CATIONS, DETECTION, DETECTORS, FLUORESCENCE, COMPUTERS, CHEMICALS, TOXICITY, COHERENCE, OPTICAL IMAGES, LASERS, DEOXYRIBONUCLEIC ACIDS, LASER APPLICATIONS, INSTRUMENTATION, DATA STORAGE SYSTEMS, ANTIBODIES, GENES, MONITORS, FREE RADICALS, ANALYZERS, LIFE(BIOLOGY), VIDEO SIGNALS, CELLS(BIOLOGY), PH FACTOR, MASS STORAGE, INDUCTION SYSTEMS, PESTICIDES, RADIATION EFFECTS, NEUROTOXINS, FLUORESCENT ANTIBODY TECHNIQUES
Field(s)/Group(s):
Toxicology, Test Facilities, Equipment and Methods
Keyword(s):
*LASER CYTOMETERS, ONCOGENES, HEPTACHLOR, PE61104D, WUAFOSR3842A4
Report Date:
1990 Jan 18