Peptide Analysis Using Tandem Mass Spectrometry

reportActive / Technical Report | Accession Number: ADA211155 | Open PDF

Abstract:

The objective of the project was to determine the complete amino acid sequence of the large polypeptide Ubiquitin by use of fast atom bombardment FAB ionization and tandem mass spectrometry. The peptide containing 76 amino acid residues was available at Warwick University as 12 tryptic digests which had previously been separated by off-line reverse phase HPLC. Most of the peptides resulting from tryptic digest contained four ten amino acid residues and were of molecular weights below 2,000 daltons. Keywords Amino acid sequences.

Author(s):
Jennings, Keith R. ; Dorwin, Ellen L.
Author Organization(s):
WARWICK UNIV COVENTRY (UNITED KINGDOM) DEPT OF CHEMISTRY
Descriptive Note:
Final rept.
Pagination:
0020

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release
Distribution Statement:
Approved For Public Release; Distribution Is Unlimited.

RECORD

Collection: TR
Identifying Numbers
Report Number(s):
R/D-6173-BC-09
Contract/Grant Number(s):
DAJA45-89-M-0041
Monitor Series:
6173-BC-09, ERO
 Subject Terms
Joint Capability Areas:
JCA_6.3.3_Spectrum Management; JCA_6_Net Centric; JCA_6.3_Net Management; JCA_3.2_Engagement; JCA_3_Force Application; JCA_3.2.1_Kinetic Means; JCA_1.2_Force Preparation; JCA_1_Force Support; JCA_1.2.7_Experimentation; JCA_6.1_Information Transport; JCA_1.2.6_Concepts; JCA_1.2.3_Educating; JCA_5.3_Planning; JCA_5_Command and Control
Communities of Interest:
Energy and Power Technologies
Descriptor(s):
*PEPTIDES, *AMINO ACIDS, REVERSIBLE, MASS SPECTROMETRY, RESIDUES, OFFLINE SYSTEMS, SEQUENCES, MOLECULAR WEIGHT, IONIZATION
Field(s)/Group(s):
Genetic Engineering and Molecular Biology
Report Date:
1989 Jun 01