Cloning and Characterization of the RecA Gene of Aquaspirillum magnetotacticum

reportActive / Technical Report | Accession Number: ADA210231 | Open PDF

Abstract:

The recA gene of Aquaspirillum magnetotacticum has been isolated from a genomic library and introduced into a recA mutant strain of Escherichia coli K12. The cloned gene complemented both the recombination and DNA repair deficiency of the host and its protein product promoted the proteolytic cleavage of the LexA protein. A protein whose molecular weight is similar to that of the RecA protein of E. coli was associated with the cloned sequence.

Author(s):
Berson, Amy E. ; Hudson, Debra V. ; Waleh, Nahid S.
Author Organization(s):
SRI INTERNATIONAL MENLO PARK CA MOLECULAR BIOLOGY DEPT
Pagination:
0021

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release
Distribution Statement:
Approved For Public Release; Distribution Is Unlimited.

RECORD

Collection: TR
Identifying Numbers
Monitor Series:
NAVEXOS
 Subject Terms
Joint Capability Areas:
JCA_5.3_Planning; JCA_5_Command and Control; JCA_4.3_Maintain; JCA_1.2.7_Experimentation
Communities of Interest:
No COI(s) Identified
Descriptor(s):
*MAGNETIC PROPERTIES, *GENES, MOLECULAR WEIGHT, DEOXYRIBONUCLEIC ACIDS, MUTATIONS, SEQUENCES, REPAIR, DEFICIENCIES, STRAINS(BIOLOGY), CLONES, RECOMBINATION REACTIONS, GENETIC ENGINEERING, PROTEINS, LIBRARIES, BACTERIA
Field(s)/Group(s):
Genetic Engineering and Molecular Biology, Microbiology
Keyword(s):
*AQUASPIRILLUM MAGNETOTACTICUM, *GENE MAPPING, MAGNETOSOMES, RECA GENE AQUASPIRILLUM MAGNETOTACTICUM GENE LIBRARY RECOMBINANT COSMID, GENE SEQUENCES, GENE MAPPING
Report Date:
1987 Jan 01