Chemical, Structural and Genetic Analysis of the Adhesive Holdfast of Biofouling Caulobacters
Abstract:
We are primarily engaged in an analysis of the adhesives that enable the stalked Caulobacter bacteria to attack and remain adherent to surfaces in the environment for long periods of time. The specific goals are 1 Determine the chemical composition and structural arrangement of monosaccharides and other substituents within the holdfasts of selected marine and freshwater Caulobacters. 2 Characterize the types of surfaces to which holdfasts will adhere. 3 Clone and analyze the genes specifying the holdfasts of selected marine and freshwater Caulobacters. 4 Develop the capabilities of selected marine Caulobacters for molecular genetic experimentation. 5 To evaluate the occurrence, stability and behavior of Caulobacters on surfaces and in complex biofilms, in collaboration with other ONR-funded researchers.