Effect of Chemical Mutagens on Herpes Virus - Induced Cellular Transformation and Testing for Mutagenesis in Mouse Cells

reportActive / Technical Report | Accession Number: ADA207137 | Open PDF

Abstract:

Assays which detect quantitative increases of morphological transformation in 3T3 cells were employed to detect transformation by seven temperature sensitive mutants of herpes simplex type 2 virus. It was determined that all seven mutants caused transformation at the non-permissive temperature. Mutant A8 293 caused formation of the most foci an appeared to be a more sensitive indicator of transformation in comparative experiments 31 foci per million cells compared to four foci per million cells for ultraviolet-irradiated wild type virus. Mutagenesis experiments using THO cells as indicator cells suggested that even after metabolic activation of the test chemicals this cell system does not provide an adequately sensitive test for mutagenesis. In additional experiments, further information was obtained which showed enhancement of transformation by hydrazine and 1,2-dimethylhydrazine of cells exposed to irradiated virus. Significant enhancement occurred in these in vitro tests only when the cells were exposed to the chemical 24 hours prior to virus infection.

Author(s):
Johnson, F. B.
Author Organization(s):
BRIGHAM YOUNG UNIV PROVO UT DEPT OF MICROBIOLOGY
Descriptive Note:
Final rept. 1 Jun 1979-31 May 1980
Pagination:
0014

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release
Distribution Statement:
Approved For Public Release; Distribution Is Unlimited.

RECORD

Collection: TR
Identifying Numbers
Report Number(s):
AFOSR-TR-89-0522
Contract/Grant Number(s):
F49620-79-C-0116
Task Number(s):
A5
Project Number(s):
2312
Monitor Series:
TR-89-0522, AFOSR
 Subject Terms
Joint Capability Areas:
JCA_1.2.7_Experimentation
Modernization Areas:
Directed Energy
Communities of Interest:
Human Systems
Descriptor(s):
*PHYSIOLOGICAL EFFECTS, *MUTAGENS, *HERPETIC VIRUSES, *MUTATIONS, MORPHOLOGY, TEST METHODS, TEST EQUIPMENT, SENSITIVITY, METABOLISM, IN VITRO ANALYSIS, IRRADIATION, MICE, VIRUS DISEASES, VIRUSES, CELLS(BIOLOGY), INDICATORS, TRANSFORMATIONS, CHEMICALS, HYDRAZINES, ACTIVATION, TEST AND EVALUATION, TEMPERATURE
Field(s)/Group(s):
Medicine and Medical Research, Toxicology, Microbiology
Keyword(s):
PE61102F, WUAFOSR2312A5
Report Date:
1980 May 31