Collection and Analysis of Complex ELISA (Enzyme-Linked Immunosorbent Assay) Data Using a Microcomputer

reportActive / Technical Report | Accession Number: ADA200869 | Open PDF

Abstract:

A system for automatic Enzyme-Linked Immunosorbent Assay ELISA data collection and processing is described. Hardware for the system consists of a Dynatch microplate reader and Apple II series microcomputer. Software developed by the authors allows automatic configuration of the reader and direct data input from the microplate reader to the microcomputer. After optical density data is input through the interface, it can be printed out as either optical density values, or as scaled values from 0 to 90 in a color-coded matrix. Data can be stored and later called in either format, allowing several sets of data to be merged into one large, color-coded chart. Results from several different microplates can be combined and sorted in a single printout to expedite interpretation of data. KeywordsBASTIC programming language Reprints Monoclonal antibodies.

Author(s):
Ross, Steven M. ; Reynolds, C. P.
Author Organization(s):
NAVAL MEDICAL RESEARCH INST BETHESDA MD
Descriptive Note:
Journal article
Supplementary Note:
Pub. in Jnl. of Immunoassay, v8 n4 p319-331 1987.
Pagination:
0014

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release
Distribution Statement:
Approved For Public Release; Distribution Is Unlimited.

RECORD

Collection: TR
Identifying Numbers
Report Number(s):
NMRI-87-92
Task Number(s):
MM33C30007, MF5852707
Project Number(s):
MM33C30, MF58527
Monitor Series:
NMC
 Subject Terms
Joint Capability Areas:
JCA_5_Command and Control; JCA_5.3_Planning
Modernization Areas:
Fully Networked C3
Communities of Interest:
Biomedical
Descriptor(s):
*DATA ACQUISITION, *INFORMATION SYSTEMS, *IMMUNOASSAY, REPRINTS, DENSITY, OPTICAL PROPERTIES, ENZYMES, OPTICAL DATA, CODING, CONFIGURATIONS, COLORS, ANTIBODIES, CLONES, MICROCOMPUTERS, CHARTS, AUTOMATIC, HIGH LEVEL LANGUAGES, INPUT, READING MACHINES, COMPUTER PROGRAMS, DATA PROCESSING
Field(s)/Group(s):
Medicine and Medical Research, Computer Programming and Software
Keyword(s):
ELISA(ENZYME LINKED IMMUNOSORBET ASSAY), BASIC PROGRAMMING LANGUAGE, PE62233N, WUDN377031
Report Date:
1987 Jan 01