Characterization of the Ptychodiscus brevis Polyether Neurotoxin Binding Component in Excitable Membranes

reportActive / Technical Report | Accession Number: ADA199525 | Open PDF

Abstract:

The research is aimed at characterization of the binding site for Ptychodiscus brevis neurotoxin PbTx-3 formerly known as T17 in nerve tissues, specifically in rat brain synaptosomes. The polyether lipid-soluble toxins isolated from the marine dinoflagellate Ptychodiscus brevis formerly Gymnodinium breve bind to a unique site, Site V, associated with the voltage- sensitive sodium channel probe for binding at Site V, dissociation constants and binding maxima have been determined in rat, fish, and turtle brain synaptosomes. Keywords Binding sites Active sites Membranes biology Sodium channels Brevetoxins Toxins.

Author(s):
Baden, Daniel G. ; Mende, Thomas J.
Author Organization(s):
MIAMI UNIV CORAL GABLES FL
Descriptive Note:
Annual rept. 1 Jun 1987-31 May 1988
Supplementary Note:
DOI: 10.21236/ADA199525
Pagination:
0029

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release

RECORD

Collection: TR
Identifying Numbers
Contract/Grant Number(s):
DAMD17-85-C-5171
Task Number(s):
AA
Project Number(s):
3M161102BS12
Monitor Series:
USAMRDC
 Subject Terms
Joint Capability Areas:
JCA_1.2.7_Experimentation; JCA_5_Command and Control; JCA_1_Force Support; JCA_6_Net Centric; JCA_1.2.5_Lessons Learned; JCA_1.3_Human Capital Management; JCA_5.3_Planning; JCA_6.1_Information Transport; JCA_1.2_Force Preparation; JCA_1.3.2_Personnel Management; JCA_6.4.1_Secure Information Exchange; JCA_4.5.4_Requirements Definition; JCA_4.5_Operational Contract Support
Communities of Interest:
Biomedical
Descriptor(s):
*MEMBRANES(BIOLOGY), *TOXINS AND ANTITOXINS, RATS, EXCITATION, SODIUM, BINDERS, DISSOCIATION, CHANNELS, NERVES, DINOFLAGELLATA
Field(s)/Group(s):
Genetic Engineering and Molecular Biology, Toxicology
Keyword(s):
*BINDING SITES, *BREVETOXINS, PTYCHODISCUS BREVIS NEUROTOXIN, ACTIVE SITES, SODIUM CHANNELS, NEUROTOXINS, PE61102A, WU105, ASS12
Report Date:
1988 Jul 31