Protein Purification and Its Application to Crystallization

reportActive / Technical Report | Accession Number: ADA199285 | Open PDF

Abstract:

Structural studies on proteins depend on an investigator s ability to isolate and purify the protein. In many cases, protein isolation is a trivial matter, but purification to homogeneity is a lengthy process. Traditionally, proteins have been purified by a combination of precipitation, open-column chromatography, including size-exclusion and ion-exchange chromatography, ultracentrifugation, and electrophoresis. Recently, high-performance liquid chromatographyHPLChas become increasingly important in the purification and analysis of proteins. The purpose of this study was to apply modern HPLC techniques to the protein purification problems encountered in crystallographic studies.

Author(s):
Deschamps, Jeffrey R.
Author Organization(s):
AMERICAN SOCIETY FOR ENGINEERING EDUCATION WASHINGTON DC
Descriptive Note:
Final rept.
Supplementary Note:
DOI: 10.21236/ADA199285
Pagination:
0046

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release
Distribution Statement:
Approved For Public Release; Distribution Is Unlimited.

RECORD

Collection: TR
Identifying Numbers
Contract/Grant Number(s):
N00014-88-J-1072
Monitor Series:
ONT
 Subject Terms
Joint Capability Areas:
JCA_1.2.7_Experimentation; JCA_5.3_Planning; JCA_5_Command and Control; JCA_1_Force Support; JCA_1.3_Human Capital Management; JCA_1.3.2_Personnel Management; JCA_6_Net Centric; JCA_JCA_4.1.2_Sustain the Force; JCA_4.1_Deployment and Distribution; JCA_4.6_Engineering; JCA_6.4.1_Secure Information Exchange; JCA_1.2_Force Preparation; JCA_4.5.4_Requirements Definition; JCA_4.5_Operational Contract Support; JCA_6.1_Information Transport
Communities of Interest:
No COI(s) Identified
Descriptor(s):
*LIQUID CHROMATOGRAPHY, *PROTEINS, *PURIFICATION, *CRYSTALLIZATION, CRYSTALLOGRAPHY, ION EXCHANGE, HOMOGENEITY, ELECTROPHORESIS, ULTRACENTRIFUGES, ISOLATION, CRYSTAL STRUCTURE, PRECIPITATION
Field(s)/Group(s):
Biochemistry, Genetic Engineering and Molecular Biology, Organic Chemistry
Report Date:
1988 Aug 30