Pathophysiology and Toxicokinetic Studies of Blue-Green Algae Intoxication in the Swine Model

reportActive / Technical Report | Accession Number: ADA192177 | Open PDF

Abstract:

Neurotoxin production is being scaled up purification methods are being compared. Mouse bioassay is used to identify toxic fractions. Stability studies with anatoxin-as revealed toxin degradation, particularly in organic solvents the process can be slowed by acidification. TLC has revealed previously unrecognized contaminants. We anticipate purification of toxin in its free base form, with normal phase HPLC because of negligible UV absorption, new methods of detection are needed. Inhibition by anatoxin-as of eel and human erythrocyte acetylcholinesterases was time and concentration dependent and followed first order kinetics, indicative of reversible binding followed by irreversible inhibition. RBC cholinesterase was more sensitive. Inhibition of horse serum cholinesterase, however, was non-linear.

Author(s):
Beasley, Val R. ; Haschek-hock, Wanda M. ; Carmichael, Wayne W. ; Cook, William O. ; Dahlem, Andrew M.
Author Organization(s):
ILLINOIS UNIV AT URBANA
Descriptive Note:
Annual rept. 1 Sep 1985-31 Aug 1986
Pagination:
0195

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release

RECORD

Collection: TR
Identifying Numbers
Contract/Grant Number(s):
DAMD17-85-C-5241
Task Number(s):
AA
Project Number(s):
3M161102BS12
Monitor Series:
USAMRDC
 Subject Terms
Joint Capability Areas:
JCA_5_Command and Control; JCA_5.3_Planning; JCA_1_Force Support; JCA_1.2_Force Preparation; JCA_1.2.3_Educating; JCA_4.5.4_Requirements Definition; JCA_4.5_Operational Contract Support; JCA_4.6_Engineering; JCA_4.6.1_General Engineering; JCA_3_Force Application; JCA_6_Net Centric; JCA_1.2.5_Lessons Learned; JCA_3.2_Engagement; JCA_8_Building Partnerships; JCA_1.2.7_Experimentation; JCA_1.4_Force Support; JCA_1.4.1_Force Health Protection; JCA_1.3_Human Capital Management; JCA_3.2.1_Kinetic Means; JCA_1.3.2_Personnel Management; JCA_8.1.1_Inform Domestic and Foreign Audiences
Modernization Areas:
Fully Networked C3
Communities of Interest:
No COI(s) Identified
Descriptor(s):
*REACTION KINETICS, *PATHOPHYSIOLOGY, *PHYTOTOXINS, *ALGAE, METHODOLOGY, STABILITY, DETECTION, ULTRAVIOLET RADIATION, PRODUCTION, MODELS, HUMANS, TOXICITY, PURIFICATION, KINETICS, ABSORPTION, MICE, ORGANIC SOLVENTS, BLOOD SERUM, ERYTHROCYTES, BLUE(COLOR), ACETYLCHOLINESTERASE, GREEN(COLOR), CHOLINESTERASE, TOXINS AND ANTITOXINS, IRREVERSIBLE PROCESSES, CNS DEPRESSANTS, BIOASSAY, SWINE
Field(s)/Group(s):
Biochemistry, Medicine and Medical Research, Toxicology
Keyword(s):
*NEUROTOXINS, PE61102A, WU006, ASS12
Report Date:
1986 Nov 21