Control of Molecular Weight Distribution of the Biopolymer Pullulan Produced by the Fungus Aureobasidium Pullulans

reportActive / Technical Report | Accession Number: ADA191040 | Open PDF

Abstract:

This report summarizes one section of this study, the evaluation of environmental conditions and their influence on the biopolymer pullulan molecular weight distribution and yield. Films, fibers, and chemical derivatives made from these different molecular weight products, and the characterization of these materials will provide the bases for another report. In order to determine optimum growth conditions for the control of molecular weight distribution of extracellular pullulan, preliminary studies utilized cell suspensions of nine strains of Aureo basidium pullulans. One strain, NRRL-Y 6220 A. pullulans, was selected for further study. Carbon and nitrogen sources, along with phosphate concentration were evaluated for their effect on pullulan yield and molecular weight distribution. Batch systems, scale-up batch, and including solvents, extraction time, etc., were also studied. Pullulan biopolymer products with weight average molecular weights from 100 thousand to 4 million, with a dispersity of around two, were produced. The evaluation of chemicalphysical properties of defined molecular weight fractions of pullulan is now under investigation. Keywords Fungus Nutrients Polysaccharides.

Author(s):
Wiley, B. J. ; Arcidiacono, S. ; Sousa, S. ; Mayer, J. M. ; Kaplan, D. L.
Author Organization(s):
ARMY NATICK RESEARCH AND DEVELOPMENT CENTER MA
Descriptive Note:
Final rept. 1986-1987
Pagination:
0030

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:
Approved For Public Release, Document Partially Illegible
Distribution Statement:
Approved For Public Release; Distribution Is Unlimited. Document Partially Illegible.

RECORD

Collection: TR
Identifying Numbers
Report Number(s):
NATICK/TR-88/012
Task Number(s):
07
Project Number(s):
1L161101A91A
Monitor Series:
NATICK
 Subject Terms
Joint Capability Areas:
JCA_8.1.1_Inform Domestic and Foreign Audiences; JCA_8.1_Communicate; JCA_8_Building Partnerships; JCA_1_Force Support; JCA_6_Net Centric; JCA_1.2_Force Preparation; JCA_3.2_Engagement; JCA_3_Force Application
Modernization Areas:
Cyber
Communities of Interest:
No COI(s) Identified
Descriptor(s):
*FUNGI, *MOLECULAR WEIGHT, *POLYSACCHARIDES, CARBON, CHEMICAL DERIVATIVES, CHEMICAL PROPERTIES, DISTRIBUTION, ENVIRONMENTS, EXTRACTION, GROWTH(GENERAL), NITROGEN, NUTRIENTS, OPTIMIZATION, PHOSPHATES, PHYSICAL PROPERTIES, SOLVENTS
Field(s)/Group(s):
Biochemistry
Keyword(s):
*PULLULAN, GLUCANS, GLUCAN ALPHA, BIOPOLYMES, AUREOBASIDIUM PULLULANS, PE61101A, WU144
Report Date:
1987 Oct 01