Wound Coverage by Cultured Skin Cells.

The aim was to confirm and extend in vitro and in vivo studies reported last year and develop new means for wound treatment. For the studies in vitro we used different tissue culture techniques. Quantitative data were obtained by cell counts and incorporation of radioactively labeled precursors, and qualitative data by light and electron microscopy. Studies in vivo were performed on experimental animals - swine, horses and dogs. Careful visual observations were documented histologically and by photography. They were supplemented by immunological approaches such as immuno-rosetting and immunoprecipitation. Keywords Collagen medium Epidermal cells Antigens Dermis Wound healing.