Structure and Functional Studies of DEN-2 Virus Genome.

Dengue-2 RNA was extracted from the virus particles. Poly A tail was added to the 3 end of DEN-2 RNA using E. coli poly A polymerase and ATP as well as T sub 4 RNA ligase and Oligo A. The poly A tailed DEN-2 was used as a template for complementary DNA synthesis using oligo dT as primer and reverse transcriptase. The size of the cDNA synthesized was determined by alkaline agarose gel electrophoresis using standard DNA marker fragments. The size is heterogeneous as expected and it falls in the range of 800 to 5000 nucleotides.