Isoelectric Focusing Patterns of Staphylococcal Exfoliative Toxin

Johnson-winegar, Anna D.; Spero, Leonard

Isoelectric Focusing Patterns of Staphylococcal Exfoliative Toxin

Active / Technical Report | Accession Number: ADA133778 |

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Abstract:

Multiple components in two antigenic variants of staphylococcal exfoliative toxin were demonstrated by isoelectric focusing in polyacrylamide gel. The main components had an isoionic point of pH 7.0 while other components ranged from pI 4.5 to pI 8.5. Each individual component was shown to possess full biologic and serologic activity after focusing. Isoelectric focusing in the presence of 8 M urea also showed microheterogeneity in exfoliative toxin preparations. The various components appear to be reversible conformers, possibly raised by exposure to the pH gradient of the ampholines.

Author(s):

Johnson-winegar, Anna D. ; Spero, Leonard

Author Organization(s):

ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD

Descriptive Note:

Journal article

Supplementary Note:

Published in Current Microbiology, v8 p311-315, 1983.

Pagination:

0005

Security Markings

DOCUMENT & CONTEXTUAL SUMMARY

Distribution:

Approved For Public Release

Distribution Statement:

Approved For Public Release; Distribution Is Unlimited.

RECORD

Collection: TR

Identifying Numbers

Monitor Series:

USAMRIID

Subject Terms

Communities of Interest:

Biomedical

Descriptor(s):

*BACTERIAL TOXINS, *STAPHYLOCOCCUS AUREUS, GELS, ISOLATION, PATTERNS, REPRINTS, SEROLOGY, UREA

Field(s)/Group(s):

Microbiology

Keyword(s):

ISOELECTRIC FOCUSING, AMPHOLINES

Report Date:

1982 Jan 01